Matches in SemOpenAlex for { <https://semopenalex.org/work/W3139795097> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W3139795097 abstract "Objective: To investigate the growth inhibition effect,cytotoxicity and apoptotic induction of harringtonine(HT) in human acute promyelocytic leukemia(APL) NB4 cells,and the related mechanism.Methods: NB4 cells were treated with HT.Total cell numbers were counted by hemocytometer,and cell viabilities were determined by trypan blue exclusion.Apoptotic cells were determined by fluorescence microscopy and FACS after staining with AO and EB or PI,respectively.The cleavage of PARP and the activation of Bax and the expression of anti-apoptotic proteins were determined by Western Blot.siRNA was used to silence the expression of target genes.Primary cells were isolated following Ficoll-Hypaque density gradient centrifugation method.Results: HT inhibited cell growth and induced apoptosis of NB4 cells in a dose-and time-dependent manner.Apoptosis induced by HT was correlated with the down-regulation of Mcl-1 and the cleavage of PARP,while HT did not affect the protein level of Bax and Bak or change the protein level of Bcl-2.The silence of Bcl-X L sensitized HT-induced apoptosis in NB4 cells.Apoptosis induced by HT in primarily cultured APL cells was also correlated with the down-regulation of Mcl-1.Conclusion: HT inhibits cell growth and induces apoptosis in NB4 cells and primarily cultured APL cells,which may be associated with down-regulation of Mcl-1." @default.
- W3139795097 created "2021-04-13" @default.
- W3139795097 creator A5005551542 @default.
- W3139795097 date "2013-01-01" @default.
- W3139795097 modified "2023-09-26" @default.
- W3139795097 title "Harringtonine induces apoptosis in NB4 cells through down-regulation of Mcl-1" @default.
- W3139795097 hasPublicationYear "2013" @default.
- W3139795097 type Work @default.
- W3139795097 sameAs 3139795097 @default.
- W3139795097 citedByCount "0" @default.
- W3139795097 crossrefType "journal-article" @default.
- W3139795097 hasAuthorship W3139795097A5005551542 @default.
- W3139795097 hasConcept C104317684 @default.
- W3139795097 hasConcept C1491633281 @default.
- W3139795097 hasConcept C153911025 @default.
- W3139795097 hasConcept C185592680 @default.
- W3139795097 hasConcept C190283241 @default.
- W3139795097 hasConcept C2776415932 @default.
- W3139795097 hasConcept C2780070996 @default.
- W3139795097 hasConcept C54009773 @default.
- W3139795097 hasConcept C54355233 @default.
- W3139795097 hasConcept C55493867 @default.
- W3139795097 hasConcept C62112901 @default.
- W3139795097 hasConcept C81885089 @default.
- W3139795097 hasConcept C86803240 @default.
- W3139795097 hasConcept C95444343 @default.
- W3139795097 hasConceptScore W3139795097C104317684 @default.
- W3139795097 hasConceptScore W3139795097C1491633281 @default.
- W3139795097 hasConceptScore W3139795097C153911025 @default.
- W3139795097 hasConceptScore W3139795097C185592680 @default.
- W3139795097 hasConceptScore W3139795097C190283241 @default.
- W3139795097 hasConceptScore W3139795097C2776415932 @default.
- W3139795097 hasConceptScore W3139795097C2780070996 @default.
- W3139795097 hasConceptScore W3139795097C54009773 @default.
- W3139795097 hasConceptScore W3139795097C54355233 @default.
- W3139795097 hasConceptScore W3139795097C55493867 @default.
- W3139795097 hasConceptScore W3139795097C62112901 @default.
- W3139795097 hasConceptScore W3139795097C81885089 @default.
- W3139795097 hasConceptScore W3139795097C86803240 @default.
- W3139795097 hasConceptScore W3139795097C95444343 @default.
- W3139795097 hasLocation W31397950971 @default.
- W3139795097 hasOpenAccess W3139795097 @default.
- W3139795097 hasPrimaryLocation W31397950971 @default.
- W3139795097 hasRelatedWork W2004367452 @default.
- W3139795097 hasRelatedWork W2161262750 @default.
- W3139795097 hasRelatedWork W2338510075 @default.
- W3139795097 hasRelatedWork W2348291000 @default.
- W3139795097 hasRelatedWork W2358888391 @default.
- W3139795097 hasRelatedWork W2370862580 @default.
- W3139795097 hasRelatedWork W2376164583 @default.
- W3139795097 hasRelatedWork W2381951882 @default.
- W3139795097 hasRelatedWork W2385748813 @default.
- W3139795097 hasRelatedWork W2390772360 @default.
- W3139795097 hasRelatedWork W2391627900 @default.
- W3139795097 hasRelatedWork W2411853640 @default.
- W3139795097 hasRelatedWork W2521385183 @default.
- W3139795097 hasRelatedWork W2778241216 @default.
- W3139795097 hasRelatedWork W2787669141 @default.
- W3139795097 hasRelatedWork W2993321364 @default.
- W3139795097 hasRelatedWork W3029635965 @default.
- W3139795097 hasRelatedWork W3029857048 @default.
- W3139795097 hasRelatedWork W3031177027 @default.
- W3139795097 hasRelatedWork W3144096873 @default.
- W3139795097 isParatext "false" @default.
- W3139795097 isRetracted "false" @default.
- W3139795097 magId "3139795097" @default.
- W3139795097 workType "article" @default.