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- W3139829920 abstract "琥珀酸 semialdehyde reductase (SSAR ) 是涉及 -aminobutyrate (伽马氨基丁酸) 的重要的酶新陈代谢。由琥珀酸把 semialdehyde (SSA ) 变换成 -hydroxybutyrate (GHB ) ， SSAR 为伽马氨基丁酸降级便于一条其他的小径。在这研究，我们从 Geobacter sulfurreducens 和 Geobacter metallireducens 识别了 SSAR (GsSSAR 和 GmSSAR，分别地) 。酶在 Escherichia coli 是过去表示的并且净化了接近同质。GsSSAR 和 GmSSAR 显示出作为一个余因子用菸碱腺嘌 dinucleotide 磷酸盐减少 SSA 的活动。oligomeric GsSSAR 和 GmSSAR 缩放，由分析尺寸排除层析决定了建议酶大概在答案作为 tetramers 存在。recombinant GsSSAR 和 GmSSAR 面对 NADP+ 结晶，并且产生晶体使衍射到 1.89 吗？(GsSSAR ) 并且 2.25？(GmSSAR ) 分辨率。GsSSAR 和 GmSSAR 晶体属于空间组 P21221 (a= 99.61 ? ， b= 147.49 ? ， c= 182.47 ?) 并且 P1 (a= 75.97 ? ， b= 79.14 ? ， c= 95.47 ? ， =82.15/" @default.
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- W3139829920 date "2011-01-01" @default.
- W3139829920 modified "2023-09-26" @default.
- W3139829920 title "Identification of succinic semialdehyde reductases from Geobacter： expression, purification, crystallization, preliminary functional, and crystallographic analysis" @default.
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