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- W3139832269 abstract "Objective: To synthesize the new insoluble 4/3 type α-conotoxin Eb1.3 mutant EB1.3[ΔR1,W13M] and determinate its binding ability to neuronal nicotinic acetylcholine receptor(nAChR) subtypes. Methods: The Eb1.3[ΔR1,W13M] linear peptide was synthesized by Fmoc-solid phase synthesis. The air oxidative folding and sulfonated modification folding were used to get aim products. The two-step oxidative folding method was then em?ployed to determine the disulfide bridge connections. Two electrode voltage clamp technique was used to deter?mine its binding to neuronal nicotinic acetylcholine receptor subtypes. Results: The disulfide-bridge framework of the main folding product of Eb1.3[ΔR1,W13M] linear peptide is a rare C1-C4,C2-C3 but not the typical C1-C3, C2-C4. The folding of sulfonated liner peptide of Eb1.3[ΔR1,W13M] was fast in the presence of reduced and oxi?dized glutathione. The inhibition ratio of Eb1.3[ΔR1,W13M] with nAChR α3β2 subtype is 28.97%±8.44% at 10μmol/L. Conclusion: Eb1.3[ΔR1,W13M] has a unique disulfide-bridge framework C1-C4,C2-C3 and is active to nAChR α3β2 subtype." @default.
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- W3139832269 date "2013-08-03" @default.
- W3139832269 modified "2023-09-24" @default.
- W3139832269 title "一种新4/3型α-芋螺毒素突变体的合成及功能研究" @default.
- W3139832269 hasPublicationYear "2013" @default.
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