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- W3139905324 abstract "To obtain flanking sequence of T-DNA tag insertion in genome is a stra tegy for investigating functional genomics by using reverse genetics. In this s tudy the plasmid rescue and tail-PCR methods were compared for their efficiency to obtain the flanking sequences T-DNA tag insertion in rice(Oryza sativa ) geno me. The results from T-DNA insertion lines showed that the efficiency of plasmid rescue was about 90%, and that of TAIL-PCR was 62%. About 80% of flanking seque nce of T-DNA tag insertion in rice genome by plasmid rescue was rice genome, whi le about 89% by TAIL-PCR. Therefore the efficiency of obtaining flanking sequenc e of T-DNA tag insertion from transgenic plants by plasmid rescue was about 72%, which was 18% higher than that by TAIL-PCR." @default.
- W3139905324 created "2021-04-13" @default.
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- W3139905324 date "2004-01-01" @default.
- W3139905324 modified "2023-09-25" @default.
- W3139905324 title "Comparison of the Efficiency Between Plasmid Rescue and TAIL-PCR Methods for Obtaining Flanking Sequence of T-DNA Tag Insertion in Rice Genome" @default.
- W3139905324 hasPublicationYear "2004" @default.
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