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- W3139930093 abstract "individual viruses to supported lipid membranes. Imaging individual virus fusion events enables hemifusion kinetics to be differentiated from pore formation kinetics. Our assays are carried out in high-throughput microfluidic devices where fusion is initiated by reducing the pH in the device. Hemifusion lag times are determined for each individual fusing virus and from this data we can determine hemfusion rate constants (kH) and the number of steps in the hemifusion pathway (N). By using a distinct two-fluorophore labeling approach, we can also measure the time to pore formation of individual viruses following the hemifusion event. In this work, we compare fusion characteristics of different strains of influenza, including seasonal and pandemic strains, and show that there are significant differences in kinetics among them." @default.
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- W3139930093 date "2012-01-01" @default.
- W3139930093 modified "2023-09-23" @default.
- W3139930093 title "Biological Large Scale Integration" @default.
- W3139930093 hasPublicationYear "2012" @default.
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