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- W3140048142 abstract "3-Aminopropyl triethoxy silane was used to modify the surface of iron oxide magnetic nanoparticles in this study.Glutaraldehyde was used as the cross-linking agent to immobilize alkaline protease.The results showed that the surface of modified magnetic nanoparticles had a good capability of magnetic separation so that alkaline protease was immobilized on its surface well.The best immobilization conditions for alkaline protease were alkaline protease amount of 7000 U/g,reaction temperature of 40 ℃ and reaction time of 1.5 h.The enzymatic activity after immobilization was 3352 U/g and the activity recovery was 48%.Moreover,immobilized alkaline protease retained 34.2% of the initial activity after fifth repeated use." @default.
- W3140048142 created "2021-04-13" @default.
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- W3140048142 date "2012-01-01" @default.
- W3140048142 modified "2023-09-24" @default.
- W3140048142 title "Immobilization of Alkaline Protease with Magnetic Nanoparticles Modified by Amino-Silane" @default.
- W3140048142 hasPublicationYear "2012" @default.
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