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• W3140094495 abstract "Abstract A simple, rapid and highly sensitive method for detection of double‐stranded DNA (dsDNA) was developed using a novel fluorescence probe composed of a RecA–GFP fusion protein that had specific recognition of ssDNA complexes (RecA–GFP–DNA filament). The RecA–GFP fusion protein not only had strong fluorescence, but could also occur by homologous recombination. In the presence of the target dsDNA, the complementary ssDNA of the RecA–GFP–DNA filaments invaded one end of the dsDNA chain. In addition, the other end of the ssDNA dissociated the RecA–GFP filaments. An assay of the probe showed a linear relationship with dsDNA concentration in the range 1–11 nM, with a correlation coefficient of 0.9923. The limit of detection for dsDNA was determined experimentally to be 0.3 nM (3δ). Compared with conventional methods, this method has the advantages of simple operation, high specificity, and high sensitivity." @default.
• W3140094495 created "2021-04-13" @default.
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• W3140094495 date "2021-04-27" @default.
• W3140094495 modified "2023-10-17" @default.
• W3140094495 title "Simple and sensitive detection of deoxyribonucleic acid using a RecA−GFP fusion protein–DNA filament as probe" @default.
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• W3140094495 doi "https://doi.org/10.1002/bio.4053" @default.
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• W3140094495 hasPublicationYear "2021" @default.
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