FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3140117453> ?p ?o ?g. }

• W3140117453 abstract "Enterotoxigenic Escherichia coli (ETEC) is one of the most common bacterial causes of mortalities in developing countries due to diarrhea. Since mucosal immune responses to CFs can prevent the disease, a chimeric protein containing ETEC's CFA/I (CfaE) tip subunits and CS2 (CotD) sub-structural units is developed to produce effective vaccine. Using bioinformatics tools, the chimeric construct was analyzed and then the optimized gene was synthesized and expressed in E. coli. The recombinant protein was expressed and purified by the Ni-NTA chromatography column and confirmed by anti-his tag antibody by western blotting. Mice were immunized with recombinant protein, and the IgG and IgA antibodies' titrations of the sera were analyzed by ELISA. In addition, the immunogenicity and protective efficacy against the live ETEC bacteria in the challenge test were determined. Western blot analysis verified the chimeric protein expression of CotD-CfaE. The outcome of ELISA was a substantial improvement in the IgG antibody titer in immunized mice. In a live ETEC challenge, the survival percentage of 30% was shown for immunized mice. The developed recombinant chimeric protein could be suggested as an effective component in producing an efficient vaccine against Enterotoxigenic E. coli with other crucial subunits, different immunization route, and other factors." @default.
• W3140117453 created "2021-04-13" @default.
• W3140117453 creator A5027848929 @default.
• W3140117453 creator A5031591136 @default.
• W3140117453 creator A5036784524 @default.
• W3140117453 creator A5057594078 @default.
• W3140117453 creator A5064617965 @default.
• W3140117453 creator A5068895763 @default.
• W3140117453 date "2021-04-09" @default.
• W3140117453 modified "2023-10-16" @default.
• W3140117453 title "Designing and determining immunogenicity of a recombinant protein due to producing a new vaccine against Enterotoxigenic Escherichia coli containing CfaE and CotD subunits" @default.
• W3140117453 cites W1898358747 @default.
• W3140117453 cites W1965770299 @default.
• W3140117453 cites W1983514420 @default.
• W3140117453 cites W1995997417 @default.
• W3140117453 cites W1998152008 @default.
• W3140117453 cites W2010041726 @default.
• W3140117453 cites W2029653875 @default.
• W3140117453 cites W2055428730 @default.
• W3140117453 cites W2067763619 @default.
• W3140117453 cites W2082792759 @default.
• W3140117453 cites W2094019736 @default.
• W3140117453 cites W2094201044 @default.
• W3140117453 cites W2095129409 @default.
• W3140117453 cites W2097874743 @default.
• W3140117453 cites W2107774571 @default.
• W3140117453 cites W2112053996 @default.
• W3140117453 cites W2113393367 @default.
• W3140117453 cites W2114676079 @default.
• W3140117453 cites W2118817344 @default.
• W3140117453 cites W2119681870 @default.
• W3140117453 cites W2120599736 @default.
• W3140117453 cites W2131994441 @default.
• W3140117453 cites W2138738692 @default.
• W3140117453 cites W2141157874 @default.
• W3140117453 cites W2142921267 @default.
• W3140117453 cites W2151287491 @default.
• W3140117453 cites W2171782036 @default.
• W3140117453 cites W2205051529 @default.
• W3140117453 cites W2270472146 @default.
• W3140117453 cites W2530359024 @default.
• W3140117453 cites W2574843527 @default.
• W3140117453 cites W2620403329 @default.
• W3140117453 cites W2718269174 @default.
• W3140117453 cites W2752778156 @default.
• W3140117453 cites W2891808294 @default.
• W3140117453 cites W2904447914 @default.
• W3140117453 cites W4293247451 @default.
• W3140117453 doi "https://doi.org/10.1080/15321819.2021.1906890" @default.
• W3140117453 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33834940" @default.
• W3140117453 hasPublicationYear "2021" @default.
• W3140117453 type Work @default.
• W3140117453 sameAs 3140117453 @default.
• W3140117453 citedByCount "0" @default.
• W3140117453 crossrefType "journal-article" @default.
• W3140117453 hasAuthorship W3140117453A5027848929 @default.
• W3140117453 hasAuthorship W3140117453A5031591136 @default.
• W3140117453 hasAuthorship W3140117453A5036784524 @default.
• W3140117453 hasAuthorship W3140117453A5057594078 @default.
• W3140117453 hasAuthorship W3140117453A5064617965 @default.
• W3140117453 hasAuthorship W3140117453A5068895763 @default.
• W3140117453 hasConcept C104317684 @default.
• W3140117453 hasConcept C123894998 @default.
• W3140117453 hasConcept C159047783 @default.
• W3140117453 hasConcept C159654299 @default.
• W3140117453 hasConcept C203014093 @default.
• W3140117453 hasConcept C2776415932 @default.
• W3140117453 hasConcept C2777871010 @default.
• W3140117453 hasConcept C2780868878 @default.
• W3140117453 hasConcept C32611913 @default.
• W3140117453 hasConcept C40767141 @default.
• W3140117453 hasConcept C547475151 @default.
• W3140117453 hasConcept C55493867 @default.
• W3140117453 hasConcept C66999527 @default.
• W3140117453 hasConcept C86803240 @default.
• W3140117453 hasConcept C89423630 @default.
• W3140117453 hasConceptScore W3140117453C104317684 @default.
• W3140117453 hasConceptScore W3140117453C123894998 @default.
• W3140117453 hasConceptScore W3140117453C159047783 @default.
• W3140117453 hasConceptScore W3140117453C159654299 @default.
• W3140117453 hasConceptScore W3140117453C203014093 @default.
• W3140117453 hasConceptScore W3140117453C2776415932 @default.
• W3140117453 hasConceptScore W3140117453C2777871010 @default.
• W3140117453 hasConceptScore W3140117453C2780868878 @default.
• W3140117453 hasConceptScore W3140117453C32611913 @default.
• W3140117453 hasConceptScore W3140117453C40767141 @default.
• W3140117453 hasConceptScore W3140117453C547475151 @default.
• W3140117453 hasConceptScore W3140117453C55493867 @default.
• W3140117453 hasConceptScore W3140117453C66999527 @default.
• W3140117453 hasConceptScore W3140117453C86803240 @default.
• W3140117453 hasConceptScore W3140117453C89423630 @default.
• W3140117453 hasLocation W31401174531 @default.
• W3140117453 hasLocation W31401174532 @default.
• W3140117453 hasOpenAccess W3140117453 @default.
• W3140117453 hasPrimaryLocation W31401174531 @default.
• W3140117453 hasRelatedWork W11188488 @default.
• W3140117453 hasRelatedWork W12268556 @default.
• W3140117453 hasRelatedWork W18394895 @default.
• W3140117453 hasRelatedWork W19354293 @default.
• W3140117453 hasRelatedWork W21164162 @default.

• next