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- W3140156404 abstract "Daphnoretin is a bicoumarin compound isolated from a natural product, Wikstroemia indica, which has been used to treat many diseases. It has strong antiviral and anti-tumor activities. Taking the anti-tumor activity of daphnoretin as a starting point, the present study aimed to test the pro-apoptotic effect of daphnoretin and its underlying mechanism in HeLa cells. The inhibitory effects of daphnoretin on viability and proliferation of HeLa cells were determined by the MTT assay. Daphnoretin-induced apoptotic morpho- logical changes were analyzed by mitochondrial membrane potential and Hoechst staining. The num- ber and stage of apoptotic HeLa cells were determined by flow cytometry. Gene expression was determined by reverse-transcription polymerase chain reaction. Protein expression was determined by western blot. The caspase activity of HeLa cells was detected by a caspase-3 and caspase-9 colorimetric assay kit. We found that daphnoretin significantly inhibited HeLa cells' viability by the MTT assay and flow cytometry. The nuclei of the apoptotic cells exhibited strong, blue fluorescence in Hoechst staining. Bax mRNA and protein levels were increased while bcl-2 mRNA levels were decreased after daphnoretin treatment. Daphnoretin also activated both caspase-3 and cas- pase-9. These findings suggest that daphnoretin pro- motes apoptosis of HeLa cells in a mitochondria- mediated way. Daphnoretin therefore has potential to be a promising drug to treat uterine cervix cancer." @default.
- W3140156404 created "2021-04-13" @default.
- W3140156404 creator A5011532728 @default.
- W3140156404 date "2014-01-01" @default.
- W3140156404 modified "2023-09-27" @default.
- W3140156404 title "Daphnoretin-induced apoptosis in HeLa cells: a possible mitochondria-dependent pathway" @default.
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