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- W3140285 abstract "This chapter gives an overview on basic concepts of microscopical techniques that allow measuring timedependent functional signals in biological tissue and compares the performance of these techniques with respect to temporal and spatial resolution. The application of conventional widefield, confocal and twophoton laser scanning microscopy to in vivo imaging is illustrated in one example, the registration of calcium signals during neuronal activity in the fly visual system. In particular, the capacity of a two-photon microscope equipped with a laser beamsplitter that enables simultaneous excitation in multiple focal spots is evaluated. By using multifocal excitation the acquisition rate can be raised considerably when it is necessary to register signals from numerous cellular structures that are far apart from each other." @default.
- W3140285 created "2016-06-24" @default.
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- W3140285 date "2007-01-01" @default.
- W3140285 modified "2023-09-27" @default.
- W3140285 title "Bright Solutions to Get Sharp Images: Confocal and Two-Photon Fluorescence Microscopy and the Pros and Cons of New Multifocal Approaches." @default.
- W3140285 hasPublicationYear "2007" @default.
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