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- W3140358586 abstract "背景然而，在成年非人类的首领的马头鱼尾的怪兽的神经发生的存在在最近的年里被证实了从这个区域的成年神经干细胞或神经祖先房间(NPC ) 的生物性质尚待广泛地被探索。现在的工作是在从成年 cynomolgus 猴子的马头鱼尾的怪兽孤立的 vitro.Methods NPC 从成年 cynomolgus 猴子的马头鱼尾的怪兽和他们的特征的考试在 NSCs/NPCs 的扩大上调查在包含生长因素的没有浆液的媒介在 vitro 被扩展，并且然后被允许由移开有丝分裂的因素区分。NPC 和他们的区别潜力的扩大能力在主要文化期间是由 immunohistochemical 和 immunocytochemical analysis.Results 的 assayed， NPC 经历了形成在暂停正在成长的 neurospheres 的房间分割，增长和聚集。没有有丝分裂的刺激，大多数 neurospheres 遵守了文化盘子并且开始区分。最后，将近 12% 区分的房间表示了神经原特定的 marker-III-tubulin (Tuj1 ) 和 84% 表示星形细胞特定的 marker-fibrillary 酸的蛋白质(GFAP ) 。另外，一个神经干细胞标记的表示， nestin，在 NPC 并且在成年猴子马头鱼尾的怪兽的 subgranular 地区被发现，在 NPC 原来被导出的地方。从成年 cynomolgus 猴子的马头鱼尾的怪兽的结论 NPC 能在 vitro 被扩展到某程度并且能够区分进神经原和星形细胞。支持的进一步的实验在里面在成年 NPC 能作为一个有用房间模型被使用用成年干细胞学习成年神经发生和房间代替治疗以前， NPC 的 vitro 增长能力将被要求。" @default.
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- W3140358586 date "2006-01-01" @default.
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- W3140358586 title "Biological properties of neural progenitor cells isolated from the hippocampus of adult cynomolgus monkeys" @default.
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