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- W3140510392 abstract "Many studies of the endothelium have assumed equivalence between cultured confluent cells and an endothelial lining in vivo. We compared gene expression of bovine aortic endothelial cells (BAECs) in culture versus freshly isolated cells from bovine aortas. Our technique of harvesting in vivo tissue yielded cells that were endothelial by the criteria of their containing von Willebrand factor (vWF) and lacking smooth muscle alpha-actin, by both immunocytochemistry and mRNA analyses. We found that several genes are overexpressed when BAECs are placed into culture, including basic fibroblast growth factor, platelet-derived growth factor B-chain, and thrombospondin. On the other hand, message for vWF is highly expressed in vivo and at lower levels in confluent cultures. The transcripts for transforming growth factor-beta, plakoglobin, and flg (fms-like gene, FGF receptor-1) are comparable in vitro and in vivo. These results demonstrate that significant changes in gene expression occur in the transition from in..." @default.
- W3140510392 created "2021-04-13" @default.
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- W3140510392 date "1993-01-01" @default.
- W3140510392 modified "2023-09-25" @default.
- W3140510392 title "Comparison of gene expression in bovine aortic endothelium in vivo versus in vitro. Differences in growth regulatory molecules." @default.
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