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- W3140555063 abstract "Abstract Background : Bovine Tuberculosis is globally the paramount cause of death from single pathogen in cattle and other species. Rapid and explicit identification of mycobacteria is essential for the control of bovine tuberculosis. We performed a fluorescence Peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) procedure for specific detection of Mycobacterium bovis and Mycobacterium avium in bovine was optimized on cytological smears and tissue sections of bovines suspected for bovine tuberculosis. Results : PNA-FISH was performed on lung and lymph node tissues impression smears. The probes were standardized for standard bovine mycobacterial cultures at 50% formamide concentration for M.bovis and 30% formamide concentration for M.avium . All the cytological smears were positive from M.bovis probe (MTBCcy3) which was standardized at hybridization conditions of (55 o C and 40% formamide) concentrations. Results revealed 4 out of 25 were positive in tissue sections with a bright red fluorescence with cy3 filter (MTBC probe). No results were seen with (MAV TAMRA ) probe for M.avium which was standardized at hybridization conditions of (55 o C and 30% formamide) respectively. No fluorescence was seen in control tissue sections .In addition, results were compared to other commonly used detection methods like IHC and PCR by targeting esxA gene. None of the sample was found positive for M. avium . Conclusion : PNA-FISH can be used in cytological impression smears and tissue sections. It is less time consuming in diagnosis of bTB in post mortem cases than PCR." @default.
- W3140555063 created "2021-04-13" @default.
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- W3140555063 date "2020-09-10" @default.
- W3140555063 modified "2023-09-25" @default.
- W3140555063 title "Rapid Detection of M. Bovis and M. Avium in Cytological Smears and Tissue Sections by PNA-FISH" @default.
- W3140555063 doi "https://doi.org/10.21203/rs.3.rs-58877/v1" @default.
- W3140555063 hasPublicationYear "2020" @default.
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