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- W3140676598 abstract "我们孤立能降级的细菌的紧张(HC4 ) 从红水藻 Hyalosiphonia caespitosa 的一个实时标本的魏 - carrageenan。与 16S rRNA 基因定序，我们作为 Tamlana sp 识别了紧张，然后从 Tamlana sp 的一种文化净化了细胞外的魏 - carrageenase。由铵硫酸盐降水， Sephadex G-200 胶化过滤层析，和 DE 纤维素的 HC4 52 阴离子交换层析。净化的酶与 66.4 kDa 的一个分子的团在 SDS 页上产出一个单身的乐队。为魏 - carrageenase 活动的最佳的 pH 和温度分别地在 8.0 和 30 掳 C。酶在 pH 的范围上是稳定的在 45 掳 C 下面的 7.2 鈥 ?.6。酶活动被 Zn2+ 和 Cu2+ 强烈在 1 mmol/L 禁止。催化酶的反应在 7.63 mg/ml 与 Michaelis 常数(K m ) 跟随 Michaelis-Menten 动力学。由 ESIMS 和 13C-NMR 光谱学的魏 - carrageenase 的降级产品的分析显示酶降级魏 - carrageenan 在下面到魏 - neocarrabiose 硫酸盐的水平。" @default.
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- W3140676598 date "2010-01-01" @default.
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- W3140676598 title "Purification and characterization of novel κ-carrageenase from marine Tamlana sp. HC4" @default.
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