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- W3140885354 abstract "细螺旋体病是螺旋体种类引起的传染细菌的疾病。在这研究，我们克隆并且定序从 L 编码 immunodominant 蛋白质 GroEL 的基因。interrogans serovar Autumnalis 种类 N2，在 Chennai 在细螺旋体病的爆发期间从一个病人的尿被孤立，印度。这 groEL 基因另外的 leptospiral serovars 与相同(99% 类似) 的高度编码 60 kDa 的蛋白质到那些。Recombinant GroEL 是在 Escherichia coli 的 overexpressed。当没有反应从 seronegative 控制病人与 sera 被观察时， Immunoblot 分析显示从证实的细螺旋体病病人的 sera 与 recombinant GroEL 显示出强壮的反应。另外， GroEL 的 3D 结构作为模板从 Thermus thermophilus 用 chaperonin 建筑群 cpn60 被构造并且验证。结果显示了 8.35 的 Z 分数，它在对为蛋白质的期望的价值的好同意。cpn60 结构的 Ca 踪迹和 leptospiral GroEL 的预言的结构的重叠与 1.5 的 RMSD 价值显示第二等的结构元素的好同意 ? 。进一步的学习是必要的作为一个疫苗的部件为细螺旋体病的血清学的诊断并且为它的潜力评估 GroEL。" @default.
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- W3140885354 date "2011-01-01" @default.
- W3140885354 modified "2023-09-25" @default.
- W3140885354 title "Cloning, Expression, and Homology Modeling of GroEL Protein from Leptospira interrogans Serovar Autumnalis Strain N2" @default.
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