Matches in SemOpenAlex for { <https://semopenalex.org/work/W3140928639> ?p ?o ?g. }
- W3140928639 abstract "Abstract Bacterial biosensors, or bactosensors, are promising field-deployable agents for medical and environmental diagnostics. However, the lack of scalable frameworks to systematically program ligand detection limits their applications. Here we present a synthetic receptor platform, termed EMeRALD ( E ngineered M odularized R eceptors A ctivated via L igand-induced D imerization) which supports the modular assembly of sensing modules onto a high-performance, generic signaling scaffold controlling gene expression in E. coli . We applied EMeRALD to detect bile salts, a biomarker of liver dysfunction, by repurposing sensing modules from enteropathogenic Vibrio species. We improved the sensitivity and lowered the limit-of-detection of the sensing module by directed evolution. We then engineered a colorimetric bactosensor detecting pathological bile salt levels in serum from patients having undergone liver transplant, providing an output detectable by the naked-eye. The EMeRALD technology enables functional exploration of natural sensing modules and rapid engineering of synthetic receptors for diagnostics, environmental monitoring, and control of therapeutic microbes." @default.
- W3140928639 created "2021-04-13" @default.
- W3140928639 creator A5004980531 @default.
- W3140928639 creator A5020950561 @default.
- W3140928639 creator A5029579606 @default.
- W3140928639 creator A5042848868 @default.
- W3140928639 creator A5052064056 @default.
- W3140928639 creator A5055503607 @default.
- W3140928639 creator A5057411659 @default.
- W3140928639 creator A5060834050 @default.
- W3140928639 creator A5061151762 @default.
- W3140928639 creator A5065016689 @default.
- W3140928639 creator A5077109814 @default.
- W3140928639 creator A5091520408 @default.
- W3140928639 date "2021-03-31" @default.
- W3140928639 modified "2023-09-26" @default.
- W3140928639 title "A synthetic receptor platform enables rapid and portable monitoring of liver dysfunction via engineered bacteria." @default.
- W3140928639 cites W1424960567 @default.
- W3140928639 cites W1966148737 @default.
- W3140928639 cites W1981401259 @default.
- W3140928639 cites W1985883733 @default.
- W3140928639 cites W1987965851 @default.
- W3140928639 cites W1989415425 @default.
- W3140928639 cites W1992761564 @default.
- W3140928639 cites W2001426985 @default.
- W3140928639 cites W2002906231 @default.
- W3140928639 cites W2005584479 @default.
- W3140928639 cites W2007749440 @default.
- W3140928639 cites W2009189380 @default.
- W3140928639 cites W2018274907 @default.
- W3140928639 cites W2030742563 @default.
- W3140928639 cites W2044019016 @default.
- W3140928639 cites W2045990864 @default.
- W3140928639 cites W2046200242 @default.
- W3140928639 cites W2049506766 @default.
- W3140928639 cites W2057927696 @default.
- W3140928639 cites W2071197649 @default.
- W3140928639 cites W2080573631 @default.
- W3140928639 cites W2117099922 @default.
- W3140928639 cites W2120500136 @default.
- W3140928639 cites W2132698384 @default.
- W3140928639 cites W2145050940 @default.
- W3140928639 cites W2151887741 @default.
- W3140928639 cites W2169515696 @default.
- W3140928639 cites W2174688478 @default.
- W3140928639 cites W2188285942 @default.
- W3140928639 cites W2192573201 @default.
- W3140928639 cites W2471624339 @default.
- W3140928639 cites W2508177680 @default.
- W3140928639 cites W2518835549 @default.
- W3140928639 cites W2562959833 @default.
- W3140928639 cites W2581803589 @default.
- W3140928639 cites W2587209830 @default.
- W3140928639 cites W2598681194 @default.
- W3140928639 cites W2599336749 @default.
- W3140928639 cites W2606042832 @default.
- W3140928639 cites W2608163069 @default.
- W3140928639 cites W2735449683 @default.
- W3140928639 cites W2744068831 @default.
- W3140928639 cites W2756289581 @default.
- W3140928639 cites W2757646744 @default.
- W3140928639 cites W2787066724 @default.
- W3140928639 cites W2790781191 @default.
- W3140928639 cites W2793943052 @default.
- W3140928639 cites W2794330007 @default.
- W3140928639 cites W2795833336 @default.
- W3140928639 cites W2801351362 @default.
- W3140928639 cites W2805945275 @default.
- W3140928639 cites W2892180666 @default.
- W3140928639 cites W2893814198 @default.
- W3140928639 cites W2893835498 @default.
- W3140928639 cites W2899571876 @default.
- W3140928639 cites W2901876957 @default.
- W3140928639 cites W2925106348 @default.
- W3140928639 cites W2951894193 @default.
- W3140928639 cites W2953085814 @default.
- W3140928639 cites W2978647098 @default.
- W3140928639 cites W2981571950 @default.
- W3140928639 cites W2993976670 @default.
- W3140928639 cites W2995534889 @default.
- W3140928639 cites W3009620456 @default.
- W3140928639 cites W3013024485 @default.
- W3140928639 cites W3014294623 @default.
- W3140928639 cites W3044778276 @default.
- W3140928639 cites W3095888206 @default.
- W3140928639 doi "https://doi.org/10.21203/rs.3.rs-358332/v1" @default.
- W3140928639 hasPublicationYear "2021" @default.
- W3140928639 type Work @default.
- W3140928639 sameAs 3140928639 @default.
- W3140928639 citedByCount "0" @default.
- W3140928639 crossrefType "posted-content" @default.
- W3140928639 hasAuthorship W3140928639A5004980531 @default.
- W3140928639 hasAuthorship W3140928639A5020950561 @default.
- W3140928639 hasAuthorship W3140928639A5029579606 @default.
- W3140928639 hasAuthorship W3140928639A5042848868 @default.
- W3140928639 hasAuthorship W3140928639A5052064056 @default.
- W3140928639 hasAuthorship W3140928639A5055503607 @default.
- W3140928639 hasAuthorship W3140928639A5057411659 @default.
- W3140928639 hasAuthorship W3140928639A5060834050 @default.
- W3140928639 hasAuthorship W3140928639A5061151762 @default.