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- W3140974912 abstract "Objective:Bifunctional alginate lyases,which could degrade both poly α-L-guluronic acid and poly β-D-mannuronic acid,could be used to prepare alginate oligosaccharides with different structure.The aim of this research is to find and characterize a bifunctional alginate lyase-producing bacterium strain.Methods:Alginate lyase-producing bacteria were screened on the selective medium using alginate as the sole carbon source and identified by 16S rDNA sequence analysis.Detection of alginate lyase activity after SDS-PAGE was carried out to determine the number and molecular weight of alginate lyase in the culture supernatant of strain QY107.The enzymatic product of alginate was analyzed by Thin-Layer Chromatography.Results:A marine bacterium strain QY107 was screened from brown algae and identified as Vibrio sp.QY107.The highest alginate lyase activity(12.32 U/mL) occurred at 120 h.There was only one alginate lyase with a molecular weight of about 28 kDa in the culture supernatant of strain QY107.The supernatant showed activities toward both poly α-L-guluronic acid and poly β-D-mannuronic acid,and released mainly trimers from alginate.Conclusions:Vibrio sp.QY107,isolated because of its high alginate lyase production,secreted only one alginate lyase into the culture supernatant.This enzyme is a bifunctional alginate lyase and could be used to prepare trisaccharides from alginate.This enzyme might have special active site structure and be a good sample for the study of structure-function relationship to find new substrate binding and catalytic mechanisms.Preparation of alginate oligosaccharides using alginate lyase have received more and more attention because of its high efficiency and low pollution to the environment.Strain QY107 might accelerate the exploitation of marine oligosaccharides and be applied in medicine,food,agriculture and biofuel industries." @default.
- W3140974912 created "2021-04-13" @default.
- W3140974912 creator A5008920458 @default.
- W3140974912 date "2013-01-01" @default.
- W3140974912 modified "2023-09-26" @default.
- W3140974912 title "Screening and Characterization of a Bifunctional Alginate Lyase-Producing Bacterium Strain" @default.
- W3140974912 hasPublicationYear "2013" @default.
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