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- W3141138940 abstract "Wide crossing is a useful method for trans- ferring traits of interest from wild Triticeae species to wheat. In this study, Aegilops sharonensis was success- fully hybridized to three tetraploid wheat species with crossability varying from 0.86 to 1.76 %. F1 hybrids were verified by their display of intermediate character- istics in morphology, cytology, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles. The exogenous x- and y-type subunits of high-molecular- weight glutenin subunits of Ae. sharonensis were expressed in F1 hybrid seeds and showed identical electrophoretic mobilities to those originally present in Ae. sharonensis. In F2 hybrids, the x-type subunit migrated faster, indicating that a decrease in molecular mass had occurred. Cloning of the open reading frames of subunit variants revealed that the x-type subunit had protein sequence variations, including single residue mutations, and insertions and deletions involving one or more repeat motifs. The two deletions causing the size reduction of the exogenous x-subunits of Ae. sharonensis in the F2 seeds were most likely the result of slip- mismatching." @default.
- W3141138940 created "2021-04-13" @default.
- W3141138940 creator A5025818408 @default.
- W3141138940 date "2014-01-01" @default.
- W3141138940 modified "2023-09-26" @default.
- W3141138940 title "Amphidiploids between tetraploid wheat and Aegilops sharonensis Eig exhibit variations in high-molecular-weight glutenin subunits" @default.
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