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- W3141472405 abstract "Objective:To investigate the effects of interleukin-27 gene expression in human pancreatic carcinoma cells (Aspc1) and the related mechanisms.Methods:The retrovirus vector was used to transduce IL-27 gene into Aspc1 cells and stable clones were obtained by selecting with G418.The expression of IL-27p28 and IL-27EBI3 in Aspc1/IL-27 cells was detected by RT-PCR.The amount of IL-27 secreted from Aspc1/IL-27 cells was determined by ELISA.Subcutaneous injected Aspc1/IL-27,Aspc1/LXSN and Aspc1 cells into nude mice was designed to observe the growth of transplanted tumor.On the tenth day,the spleens cells were extracted and then IFN-γ,TNF-α and IL-12 were detected by ELISA.Cytolysis of spleen cells stimulated by Aspc1 cells was detected by LDH method.Results:Aspc1/IL-27 cells were successfully set up and detected the expression of IL-27 at gene and protein levels.Growth of the tumor formed by Aspc1/IL-27 cells was significantly supressed after transplantation in nude mice.Meanwhile,the spleen cells of this group secreted higher level of IFN-γ,TNF-α and IL-12 than those of the other two groups (P0.01).Cytotoxicity of spleen cells stimulated by Aspc1 cells was significantly increased (P0.01).Conclusion:These results suggest that IL-27 has antitumor activity through enhancing immonological function in vivo." @default.
- W3141472405 created "2021-04-13" @default.
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- W3141472405 date "2010-01-01" @default.
- W3141472405 modified "2023-09-23" @default.
- W3141472405 title "The antitumor activity of IL-27 gene transduced into Aspc1 cells and its mechanism in nude mouse" @default.
- W3141472405 hasPublicationYear "2010" @default.
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