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• W3141502007 abstract "High-throughput tag-sequencing (Tag-seq) from Illumina analysis, which is based on the Solexa Genome Analyzer platform, was applied to analyze the gene expression profiling of propamocarb (PM) treatment and control in cucumber fruit. Approximately 3.6 million complete clean sequence tags at PM treatment or control library were obtained with approximately 0.1 million dis- tinct clean tag sequences. Approximately 41.79-43.15 % of the distinct clean tags were mapped unambiguously to the unigene database, and 32.54-33.46 % of the distinct clean tags were mapped to the cucumber genome database. The profiling analysis of the differentially expressed genes revealed the up-regulation of 546 genes and the down- regulation of 185 genes with PM response. Furthermore, the differentially expressed genes mainly linked to pesti- cide detoxication, response to stress/stimulus, transporter/ signaling, and some important transcription factors. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) and reverse transcription polymerase chain reaction (RT-PCR) using 16 genes independently verified the tag-mapped results. The present study reveals the comprehensive mechanisms of PM response in cucumber fruit." @default.
• W3141502007 created "2021-04-13" @default.
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• W3141502007 date "2013-01-01" @default.
• W3141502007 modified "2023-09-27" @default.
• W3141502007 title "Transcriptome analysis reveals differentially expressed genes associated with propamocarb response in cucumber (Cucumis sativus L.) fruit" @default.
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• W3141502007 hasPublicationYear "2013" @default.
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