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- W3141520235 abstract "调查乳的酸是否能在老鼠禁止 NF-B p65 的 LPS 激活肠的 mucosa microvascular endothelial 房间(RIMMVEC ) , RIMMVEC,在 vitro 有教养,是有乳的酸的不同集中的 pretreated 然后暴露了到 lipopolysaccharide (LPS ) 。房间和房间文化媒介然后在不同时间间隔被收集。肿瘤坏死因素的生产 --(TNF-) 并且 interleukin-6 (IL-6 ) 被连接酶的 immunosorbent 在蛋白质水平检验试金。NF-B 的 LPS 激活上的乳的酸的影响被即时量的 PCR 和西方的污点分析分别地在 mRNA 和蛋白质层次检验。TNF-6 和 IL-6 蛋白质层次显著地与仅仅暴露于 LPS 的房间相比与乳的酸在预告的处理以后被减少。在有 7.5, 5.0,和 2.5 L mL1 的预告的处理以后乳的酸,分别地,没有 LPS 处理, NF-B mRNA 层次被 1.51- , 2.62-fold, 和 3.00 褶层在控制房间与层次相比增加。西方的污点分析显示在乳的 acid-pretreated 组的 NF-B p65 的水平在仅仅与 LPS 对待的组(积极控制) 是比那显著地低的并且没有 LPS 处理(空白的控制) ,与这个组相比是未改变的。这些结果建议乳的酸可以禁止 NF-B 的 LPS 激活,导致 TNF-6 和 IL-6 的下面规定。" @default.
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- W3141520235 date "2011-01-01" @default.
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- W3141520235 title "Lactic Acid Inhibits NF-kB Activation by Lipopolysaccharide in Rat Intestinal Mucosa Microvascular Endothelial Cells" @default.
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