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- W3141536319 abstract "spermatid 特定的基因， sp3111，是 four-transmembrane 基因家庭的一个新成员。然而，它的繁殖生物功能留下逃犯。这研究的目的是为进一步在它的繁殖功能上学习 sp3111 基因表示的抑制的效果建立一个 sp3111 击倒的雄的老鼠模型。pSUPER-sp3111-shRNA 的 Recombinant 向量，对老鼠 sp3111 mRNA 的不同区域包含抄录功能的小发卡 RNA 顺序，被构造并且识别。然后，线性化的 recombinant 向量被 electroporation 注入成熟老鼠睾丸和 transfected 到 spermatogonial 房间产生 sp3111 转基因的 RNAi 老鼠。这些 electroporated 男性老鼠与野类型的女性一起被交配在 electroporation 和他们的子孙以后的 30 天被荧光显微镜学和 PCR 两个都检验。在 vivo 的 sp3111 mRNA 的睾丸表达式上的 RNAi 的抑制效率是超过30%并且能稳定地被传给 F3 产生，这被发现，并且与野类型的雄的鼠标相比，吗 sp3111 RNAi 雄的鼠标的后代的吝啬的数字从 11 被减少???????????????????????栠吗？？" @default.
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- W3141536319 date "2010-01-01" @default.
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- W3141536319 title "Generation of sp3111 transgenic RNAi mice via permanent integration of small hairpin RNAs in repopulating spermatogonial cells in vivo" @default.
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