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• W3141539845 abstract "Adaptation of assays on dried blood has advantages of ease of collection, transportation, minimal invasiveness and requirement of small volume. A method for extraction and estimation of triglyceride from blood spots dried on filter paper (Whatman no. 3) has been developed. A single dried blood spot containing 10 μL blood was used. Triglyceride was efficiently extracted in methanol from blood dried on filter paper by incubation at 37°C for two hours with gentle shaking. For the estimation, a commercially available enzymatic method was used. Blood spot assays showed mean intra and inter assay coefficient of variance of 6.0% and 7.4% respectively. A comparison of paired whole blood spots and plasma samples (n = 75, day 0) gave an intraclass correlation of 0.96. The recovery was 99.6%. The dried blood triglyceride concentrations were stable for one month when the filter discs were stored at room temperature (16–28°C). Storage of filters at 4°C extended the stability and triglycerides could be quantatively recovered after 3 months of storage. Findings Serum triglyceride (TG) is an important risk factor for heart diseases [1-3]. Large scale epidemiological evaluation of TG poses several challenges in developing countries. Storage and transportation of the samples from field is a limiting factor, especially in areas lacking modern laboratory facilities. A method that eliminates the need to store and ship samples at low temperatures would therefore be desirable. Assays using whole blood dried on filter paper may provide a viable alternative. Several community based studies have shown this to be convenient and reliable for evaluation of a number of analytes [4-8]. We describe here a method for extraction and measurement of TG from blood samples collected on Whatman no. 3 filter paper (Whatman international Ltd, England.) Seventy five patients visiting the centralized laboratory, Cardio-Neuro Center, All India Institute of Medical Sciences (AIIMS) for lipid investigations were selected at random. Blood was collected by venipuncture into tubes with anticoagulant. Ethical clearance for the conduct of the study was obtained from institutional (AIIMS) ethics committee. Blood spots were prepared by pipetting 10 μL of the blood onto the filter paper kept on a nonabsorbent surface (thermacol) and left at room temperature for drying. The room temperature was 16–28°C for the duration of study. After drying, the filter discs were kept in sealed plasPublished: 14 July 2006 Lipids in Health and Disease 2006, 5:20 doi:10.1186/1476-511X-5-20 Received: 30 May 2006 Accepted: 14 July 2006 This article is available from: http://www.lipidworld.com/content/5/1/20 © 2006 Quraishi et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited." @default.
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• W3141539845 date "2015-01-01" @default.
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• W3141539845 title "Lipids in Health and Disease" @default.
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