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- W3141570797 abstract "Objectives To quantitatively describe the adhesion of hepatocellular carcinoma (HCC) cells and hepatocytes to collagen /laminin coated surfaces and to investigate the relevance of cell adhesion forces to the coating concentration of collagen and, when laminin was cocoated along with collagen , the incorporation concentration of laminin. Methods A stepwise coating protocol was adopted to prepare the collagen /laminin coated surfaces, in which polyDlysine and collagen /laminin were successively coated onto glass surfaces and finally, bovine serum albumin was used to block the nonspecific adhesion. Cell adhesion forces were measured by means of micropipette aspiration technique. Results Cell adhesion forces showed good dependence on the coating concentration of collagen . However, the amplitude for the increase of adhesion forces of HCC cells (20210-10N,26%) was far more less than that for hepatocytes(1 56610-10N,742%) when coating concentration increased from 1 g/ml to 5 g/ml. No further increase in the adhesion forces of HCC cells was observed when the coating concentration of collagen IV increased beyond 2g/ml. The incorporation of laminin along with 2 g/ml collagen did not readily result in an increase in the adhesion forces of the two kinds of cells to the surfaces. Among the concentration of laminin tested, only in certain concentration(0.625 g/ml or1.25 g/ml) did the incorporation of laminin along with 2 g/ml collagen IV lead the adhesion forces of hepatocytes and HCC cells to the coated surfaces to reach their corresponding maximum values, otherwise this incorporation led the adhesion forces to decrease. Conclusion The present method is efficient in investigating cellmatrix adhesion. The cell adhesion behavior to collagen for HCC differs obviously from those for normal hepatocytes. Laminin might be an important factor for the regulation of cell adhesion forces to matrix components" @default.
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- W3141570797 date "1999-01-01" @default.
- W3141570797 modified "2023-09-25" @default.
- W3141570797 title "Adhesion of hepatocellular carcinoma cells to collagen coated surfaces." @default.
- W3141570797 hasPublicationYear "1999" @default.
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