FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W314162389> ?p ?o ?g. }

• W314162389 abstract "Malaysia as a tropical country is a rich source of biologically active phytochemicals, which could be useful as an alternative to the current unsafe regimens of cancer treatment. This includes the use of cisplatin (CIS), the current chemotherapeutic drug to treat cervical cancer, the second most lethal cancer affecting women in Malaysia. Therefore, anti-tumor activities of zerumbone (ZER) were investigated in both in vitro and in vivo cervical cancer models. This natural compound was isolated from the edible plant Zingiber zerumbet, locally known as Lempoyang, through column chromatography and hydrodistillation methods. The chemical structure of ZER was confirmed using NMR. The cytotoxic effects of ZER were tested in human cervical cancer cell lines (HeLa) using MTT assay and compared concurrently to cisplatin. Zerumbone’s induction of HeLa cancer cell deaths were quantified using AO/PI double staining and flow cytometry. Transmission and scanning electron microscopic analyses were done to evaluate ultra-morphological changes. The effect of ZER on caspase-3 and caspase-9 was evaluated colorimetrically in HeLa cells. The in vivo model of cervical intraepithelial neoplasia (CIN) was induced in pregnant female Balb/c mice using Diethylstilboestrol (DES). Cervical tissues were stained with hematoxylin and eosin (H&E) and viewed under light microscopy and the in vivo antiproliferative properties of ZER was confirmed by the immunohistochemical staining of proliferating cellular nuclear antigen (PCNA) as a proliferation marker and the PCNA labeling index was obtained. Apoptosis (Bcl-2 & Bax) and G2/M-cell cycle arrest (cdc25B, cyclinB1 and Chk2) associated proteins were investigated using immunohistochemistry. Moreover, RT-PCR was used to amplify mRNA of Bcl-2, Bax, c-myc and β-actin genes. The genetic material was obtained by laser capture microdissection microscopy (LCMM). No previous toxicological investigations have been carried out on this compound. Hence, acute, sub-acute and sub-chronic toxicity studies and ZER was evaluated for its behavioural, biochemical and histo-pathological effects. Findings of NMR coincide to the previously published data. However, ZER was able to exert an antiproliferative effect towards HeLa when isolated by both hydrodistillation and column chromatography, with an IC50 of 20.30±1.1 μM and 20.41±0.9 μM (p>0.05, student t-test, n=3), respectively. AO/PI-stained HeLa cells showed that ZER induced apoptosis in a time-dependent manner with insignificant statistical (p>0.05) difference in necrosis between various doses of this compound. Moreover, flow cytometric evaluation of the effect of ZER on DNA content by cell cycle phase distribution revealed that the cell populations at G0 and G2/M phases were significantly different (p 0.05) serum concentrations of AST, ALP, ALT and GGT. No histopathological changes were observed in the hepatic, renal, cardiac and gastrointestinal tissues. These histomorphological findings were supported by the insignificant differences (p>0.05) between the mean lesion scores of hepatic and renal tissues. Collectively, results presented in this study demonstrated that ZER causes metaphasal blockage in HeLa cells, leading to growth inhibition and apoptosis, which was later confirmed to be through mitochondrial pathways. As ZER exhibits similar pharmacological activity to CIS, it possesses the potential to be developed as an antiproliferative agent for cervical cancer but producing less side effects, as the compound was shown to have no toxicological signs compared to the clinical complications of CIS." @default.
• W314162389 created "2016-06-24" @default.
• W314162389 creator A5012179237 @default.
• W314162389 date "2009-01-01" @default.
• W314162389 modified "2023-09-24" @default.
• W314162389 title "Antitumor Effect of Zerumbone Isolated from Lempoyang (Zingiber Zerumbet) on Human Cervical Cancer Cells and Mouse Cervical Intraepithelial Neoplasia" @default.
• W314162389 hasPublicationYear "2009" @default.
• W314162389 type Work @default.
• W314162389 sameAs 314162389 @default.
• W314162389 citedByCount "1" @default.
• W314162389 countsByYear W3141623892013 @default.
• W314162389 crossrefType "dissertation" @default.
• W314162389 hasAuthorship W314162389A5012179237 @default.
• W314162389 hasConcept C121608353 @default.
• W314162389 hasConcept C126322002 @default.
• W314162389 hasConcept C142724271 @default.
• W314162389 hasConcept C1491633281 @default.
• W314162389 hasConcept C150903083 @default.
• W314162389 hasConcept C153911025 @default.
• W314162389 hasConcept C185592680 @default.
• W314162389 hasConcept C190283241 @default.
• W314162389 hasConcept C204232928 @default.
• W314162389 hasConcept C207001950 @default.
• W314162389 hasConcept C2776694085 @default.
• W314162389 hasConcept C2777343196 @default.
• W314162389 hasConcept C2777366897 @default.
• W314162389 hasConcept C2778220009 @default.
• W314162389 hasConcept C2778239845 @default.
• W314162389 hasConcept C2780783641 @default.
• W314162389 hasConcept C29537977 @default.
• W314162389 hasConcept C34688535 @default.
• W314162389 hasConcept C502942594 @default.
• W314162389 hasConcept C553184892 @default.
• W314162389 hasConcept C55493867 @default.
• W314162389 hasConcept C71924100 @default.
• W314162389 hasConcept C74864618 @default.
• W314162389 hasConcept C86803240 @default.
• W314162389 hasConceptScore W314162389C121608353 @default.
• W314162389 hasConceptScore W314162389C126322002 @default.
• W314162389 hasConceptScore W314162389C142724271 @default.
• W314162389 hasConceptScore W314162389C1491633281 @default.
• W314162389 hasConceptScore W314162389C150903083 @default.
• W314162389 hasConceptScore W314162389C153911025 @default.
• W314162389 hasConceptScore W314162389C185592680 @default.
• W314162389 hasConceptScore W314162389C190283241 @default.
• W314162389 hasConceptScore W314162389C204232928 @default.
• W314162389 hasConceptScore W314162389C207001950 @default.
• W314162389 hasConceptScore W314162389C2776694085 @default.
• W314162389 hasConceptScore W314162389C2777343196 @default.
• W314162389 hasConceptScore W314162389C2777366897 @default.
• W314162389 hasConceptScore W314162389C2778220009 @default.
• W314162389 hasConceptScore W314162389C2778239845 @default.
• W314162389 hasConceptScore W314162389C2780783641 @default.
• W314162389 hasConceptScore W314162389C29537977 @default.
• W314162389 hasConceptScore W314162389C34688535 @default.
• W314162389 hasConceptScore W314162389C502942594 @default.
• W314162389 hasConceptScore W314162389C553184892 @default.
• W314162389 hasConceptScore W314162389C55493867 @default.
• W314162389 hasConceptScore W314162389C71924100 @default.
• W314162389 hasConceptScore W314162389C74864618 @default.
• W314162389 hasConceptScore W314162389C86803240 @default.
• W314162389 hasLocation W3141623891 @default.
• W314162389 hasOpenAccess W314162389 @default.
• W314162389 hasPrimaryLocation W3141623891 @default.
• W314162389 hasRelatedWork W1536544722 @default.
• W314162389 hasRelatedWork W2020963110 @default.
• W314162389 hasRelatedWork W2134343852 @default.
• W314162389 hasRelatedWork W2145107307 @default.
• W314162389 hasRelatedWork W2147885817 @default.
• W314162389 hasRelatedWork W2188362851 @default.
• W314162389 hasRelatedWork W2348375949 @default.
• W314162389 hasRelatedWork W2364121332 @default.
• W314162389 hasRelatedWork W2380575839 @default.
• W314162389 hasRelatedWork W2515920880 @default.
• W314162389 hasRelatedWork W2770128573 @default.
• W314162389 hasRelatedWork W2908265669 @default.
• W314162389 hasRelatedWork W2912282151 @default.
• W314162389 hasRelatedWork W2953816119 @default.
• W314162389 hasRelatedWork W3044818023 @default.
• W314162389 hasRelatedWork W3046915674 @default.
• W314162389 hasRelatedWork W3092434513 @default.
• W314162389 hasRelatedWork W3175543386 @default.
• W314162389 hasRelatedWork W3182062534 @default.
• W314162389 hasRelatedWork W2578897791 @default.
• W314162389 isParatext "false" @default.
• W314162389 isRetracted "false" @default.
• W314162389 magId "314162389" @default.
• W314162389 workType "dissertation" @default.