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- W3141735523 abstract "Objective Some recent studies revealed that phenthiazine might be able to reverse tumor cell drug-resistance. Chlorderazin belongs to the phenthiazine compounds. The study aimed to investigate the reversing effect and mechanism of chlorderazin on multidrug resistance of leukemic cell line K562/AO2. Methods (1) The cytotoxicities of chlorderazin were assayed with the tetrazolium dye, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. (2) The reverse effect of chlorderazin on K562/AO2 cells was analyzed with MTT method. The multidrug resistance reversal index (RI)was equal to the ratio of control group IC 50/test group half inhibition concentration IC 50. (3) The intracellular daunorubicin (DNR) concentrations were measured by the flow cytometry. (4) Mdr1 mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR). The ratio of mdr-1/β-actin density was calculated. Results (1) Chlorderazin 3 μg/ml showed little toxicity to K562/AO2 cells and the suppression rate was less than 5%, so the concentration of 3 μg/ml chlorderazin was selected as the experiment concentration. (2)The cytotoxicities of DNR to K562/AO2 were enhanced by 3 μg/ml of chlorderazin (P0.05) and RI was 1.901. (3) Chlorderazin of 3 μg/ml could increase the intracellular DNR accumulation significantly (P0.05), and the fluorescence staining by the flow cytometry was higher (250.95±18.96)than the control group(112.75±15.78)and shift right in K562/AO2 cells treated with chlorderazin, and the difference was significant(P0.05). (4) Chlorderazin has no significant influence to the expression level of mdr-1 mRNA. Both test group and control group showed a clear mdr-1 mRNA band located at the position of 157kb. The ratios of mdr-1/β-actin density were 0.414±0.012 in the test group and 0.447±0.027 in the control group, respectively, and the difference was not significant(P0.05). Conclusion Chlorderazin could reverse the multidrug resistance by increasing the intracellular DNR accumulation in K562/AO2 cells. The effects had no correlation to the mdr-1 gene.Further study is needed." @default.
- W3141735523 created "2021-04-13" @default.
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- W3141735523 date "2003-01-01" @default.
- W3141735523 modified "2023-09-23" @default.
- W3141735523 title "Reversal of multidrug resistance in leukemic cell line K562/AO2 by chlordelazine in vitro" @default.
- W3141735523 hasPublicationYear "2003" @default.
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