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- W3141823674 abstract "Cd Te nanocrystals were synthesized in aqueous solution and capped with amercaptopropionic acid,which were used as a kind of fluorescent probe to label Alcalase. The fluorescence spectra showed that Cd Te nanocrystals were accompanied by a narrow size distribution and high stability. The fluorescence intensity decreased 8. 36% after 30 d at room temperature. Transmission electron microscopy( TEM) imaging revealed that Cd Te nanocrystal's diameter was about 3 nm,and X-ray diffraction( XRD) pattern showed it with zinc blende structure. After being conjugated with Alcalase using 1-ethyl-3-( 3-dimethyl aminopropye) carbodii-mide hydroclloride( EDC) and N-hydroxysulfosucinimide sodium salt( NHSS) methods,the Cd Te-Alcalase conjugates were separated and purified by ultra-filter-centrifugation,a Sephadex G-100 column and sodium dedecye sulfate( SDS)-polyacrylamide gel electrophoresis. Fluorescent spectral analysis showed that Cd Te-Alcalase conjugates also presented good optical stability compared with Cd Te nanocrystals. The enzyme activity was also relatively high at 61. 06 U / μg. The fluorescent emission of the Cd Te-Alcalase conjugates was visualized with a fluorescence microscope,which would be suitable for tracing the enzyme reaction in the future." @default.
- W3141823674 created "2021-04-13" @default.
- W3141823674 creator A5021470829 @default.
- W3141823674 date "2015-01-01" @default.
- W3141823674 modified "2023-09-23" @default.
- W3141823674 title "Labeling and Purification of Alcalase and Fluorescent Nanocrystals Synthesized in Water Phase" @default.
- W3141823674 hasPublicationYear "2015" @default.
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