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- W3141932877 abstract "Chilling stress can have severe impacts on the growth, development and productivity of maize worldwide. In the present study, cDNA amplified fragment length polymorphism (cDNA-AFLP) analysis was used to evalu- ate gene expression in maize during chilling treatments (6C) over four time periods (0, 2, 6 and 12 h). A total of 441 transcript-derived fragments (TDFs) induced by low- temperature treatment were detected. Based on the sequence analysis, the 58 TDFs of known functions were involved in metabolism, photosynthesis, signal transduc- tion and defence responses etc., suggesting that maize undergoes a complex adaptive process in response to low temperatures. Three full-length cDNA, encoding MAP- KKK (mitogen-activated protein kinase kinase kinase), CLC-D (chloride channel D) and RLK (receptor-like pro- tein kinases) homologues, were isolated from maize through in silico cloning and named as ZmMAPKKK, ZmCLC-D and ZmRLK, respectively. Finally, the expres- sion patterns of the three genes showed a significant increase of differential expression after chilling stress as analysed by semi-quantitative RT-PCR and real-time qRT- PCR. This study provides important clues to understanding low-temperature regulation mechanisms in maize and the three candidate genes involved in chilling responses need further research to determine their usefulness in breeding new resistance cultivars." @default.
- W3141932877 created "2021-04-13" @default.
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- W3141932877 date "2011-01-01" @default.
- W3141932877 modified "2023-09-27" @default.
- W3141932877 title "Identification and characterisation of candidate genes involved in chilling responses in maize (Zea mays L.)" @default.
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