FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3141933095> ?p ?o ?g. }

• W3141933095 endingPage "3113" @default.
• W3141933095 startingPage "3113" @default.
• W3141933095 abstract "3113 Background: Effective treatment of cancers using radioactive iodine is contingent upon constitutive and strong expression of NIS. Doxorubicin has been shown to induce expression of NIS in hepatocellular cancer (HCA) by activation of p63 and p73 binding to NIS promoter, leading to an increased expression of NIS mRNA and protein in HCA cells but not in primary human hepatocytes. Purpose: To demonstrate that NIS expression in HCA cells following doxorubicin treatment can be studied using immunofluorescence imaging. Methods: HepG2 cells were seeded at 1 x 105 cells per well and treated with 1 µM doxorubicin hydrochloride for 24hrs. Cells were washed in 1xPBS, fixed for 10 minutes with 4% paraformaldehyde (PFA), permeabilized for 10 mins in 0.2% Triton X-100 in PBS and blocked with 1% BSA, 22.52mg/mL glycine in PBST for 30 mins. Samples were not incubated (control) or incubated overnight at 4 deg C with rabbit anti-NIS primary antibody (Imanis Life Sciences) at 1:1000, washed 3 times with 1X PBST, and incubated with goat anti-rabbit IgG Alexa Fluor® 488 labeled secondary antibody (Santa Cruz Biotechnology) for 1 hour in dark. Coverslips were counterstained with 1 μg/mL Hoechst, mounted on slides using Vectashield medium (Vector labs, Ca) and sealed with nail polish. Fluorescence imaging was accomplished using a confocal microscope (inverted Nikon Eclipse Ti2 microscope equipped with a 60X apochromat oil-immersion OFN25 objective). Results: Our studies demonstrate that: 1) HCA cells can be viably treated with Doxorubicin. 2) NIS expression in HCA cells are recognized by anti-NIS antibodies and imaged with immunofluorescence. 3) NIS protein is expressed on the surface of HCA cells following treatment with Doxorubicin at 1μM. Conclusions: NaI symporter is expressed on the surface of hepatocellular cancer cells following doxorubicin treatment. These findings can be exploited in the use of I-131 radioisotope as a theranostic tool for hepatocellular cancers.Fig.1. Imaging of Sodium Iodide Symporter Expression Following Treatment with Doxorubicin in HepG2 Hepatocellular Cancer Monolayer Cells. HepG2 cells were treated with 1µM doxorubicin hydrochloride for 24 hours and fluorescent imaging was performed without (left) or with (right) anti-NIS 10 antibody, followed by Alexa Fluor® 488 20 antibody and Hoechst.Fig. 2. Sodium Iodide Symporter is expressed on the surface of HepG2 Hepatocellular Caner Cell Following Doxorubicin Treatment. Representative slices of Z-stack confocal imaging of NaI symporter expression in HepG2 cell following 1µM doxorubicin hydrochloride treatment. A single cell was imaged at 20X (left) and 60X (right) with anti-NIS 10 antibody, Alexa Fluor® 488 20 antibody, and Hoechst." @default.
• W3141933095 created "2021-04-13" @default.
• W3141933095 creator A5024446851 @default.
• W3141933095 creator A5037734282 @default.
• W3141933095 creator A5071799659 @default.
• W3141933095 date "2020-05-01" @default.
• W3141933095 modified "2023-09-23" @default.
• W3141933095 title "Endogenous Expression of Sodium Iodide Symporter in Hepatocellular Carcinoma Cells" @default.
• W3141933095 hasPublicationYear "2020" @default.
• W3141933095 type Work @default.
• W3141933095 sameAs 3141933095 @default.
• W3141933095 citedByCount "0" @default.
• W3141933095 crossrefType "journal-article" @default.
• W3141933095 hasAuthorship W3141933095A5024446851 @default.
• W3141933095 hasAuthorship W3141933095A5037734282 @default.
• W3141933095 hasAuthorship W3141933095A5071799659 @default.
• W3141933095 hasConcept C121332964 @default.
• W3141933095 hasConcept C153911025 @default.
• W3141933095 hasConcept C159654299 @default.
• W3141933095 hasConcept C169274487 @default.
• W3141933095 hasConcept C178790620 @default.
• W3141933095 hasConcept C185592680 @default.
• W3141933095 hasConcept C203014093 @default.
• W3141933095 hasConcept C21269302 @default.
• W3141933095 hasConcept C2776694085 @default.
• W3141933095 hasConcept C2776759294 @default.
• W3141933095 hasConcept C2778019345 @default.
• W3141933095 hasConcept C2778236600 @default.
• W3141933095 hasConcept C2778723968 @default.
• W3141933095 hasConcept C2779178360 @default.
• W3141933095 hasConcept C2781303535 @default.
• W3141933095 hasConcept C502942594 @default.
• W3141933095 hasConcept C54355233 @default.
• W3141933095 hasConcept C62520636 @default.
• W3141933095 hasConcept C86803240 @default.
• W3141933095 hasConcept C91881484 @default.
• W3141933095 hasConcept C95444343 @default.
• W3141933095 hasConceptScore W3141933095C121332964 @default.
• W3141933095 hasConceptScore W3141933095C153911025 @default.
• W3141933095 hasConceptScore W3141933095C159654299 @default.
• W3141933095 hasConceptScore W3141933095C169274487 @default.
• W3141933095 hasConceptScore W3141933095C178790620 @default.
• W3141933095 hasConceptScore W3141933095C185592680 @default.
• W3141933095 hasConceptScore W3141933095C203014093 @default.
• W3141933095 hasConceptScore W3141933095C21269302 @default.
• W3141933095 hasConceptScore W3141933095C2776694085 @default.
• W3141933095 hasConceptScore W3141933095C2776759294 @default.
• W3141933095 hasConceptScore W3141933095C2778019345 @default.
• W3141933095 hasConceptScore W3141933095C2778236600 @default.
• W3141933095 hasConceptScore W3141933095C2778723968 @default.
• W3141933095 hasConceptScore W3141933095C2779178360 @default.
• W3141933095 hasConceptScore W3141933095C2781303535 @default.
• W3141933095 hasConceptScore W3141933095C502942594 @default.
• W3141933095 hasConceptScore W3141933095C54355233 @default.
• W3141933095 hasConceptScore W3141933095C62520636 @default.
• W3141933095 hasConceptScore W3141933095C86803240 @default.
• W3141933095 hasConceptScore W3141933095C91881484 @default.
• W3141933095 hasConceptScore W3141933095C95444343 @default.
• W3141933095 hasLocation W31419330951 @default.
• W3141933095 hasOpenAccess W3141933095 @default.
• W3141933095 hasPrimaryLocation W31419330951 @default.
• W3141933095 hasRelatedWork W1641520282 @default.
• W3141933095 hasRelatedWork W2023760126 @default.
• W3141933095 hasRelatedWork W2033947583 @default.
• W3141933095 hasRelatedWork W2040525311 @default.
• W3141933095 hasRelatedWork W2056374170 @default.
• W3141933095 hasRelatedWork W2292350757 @default.
• W3141933095 hasRelatedWork W2359335963 @default.
• W3141933095 hasRelatedWork W2364755482 @default.
• W3141933095 hasRelatedWork W2378583759 @default.
• W3141933095 hasRelatedWork W2388305780 @default.
• W3141933095 hasRelatedWork W2473536083 @default.
• W3141933095 hasRelatedWork W2559884509 @default.
• W3141933095 hasRelatedWork W2769353100 @default.
• W3141933095 hasRelatedWork W2775828964 @default.
• W3141933095 hasRelatedWork W2885282682 @default.
• W3141933095 hasRelatedWork W2924087575 @default.
• W3141933095 hasRelatedWork W3029051159 @default.
• W3141933095 hasRelatedWork W3030827540 @default.
• W3141933095 hasRelatedWork W3036413853 @default.
• W3141933095 hasRelatedWork W95520772 @default.
• W3141933095 hasVolume "61" @default.
• W3141933095 isParatext "false" @default.
• W3141933095 isRetracted "false" @default.
• W3141933095 magId "3141933095" @default.
• W3141933095 workType "article" @default.