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- W3141959374 abstract "Marek 的疾病病毒(MDV ) 的完全的染色体紧张 GX0101，与加快的 LTR 序列综合，作为一个细菌的人工的染色体(用绳子拖的平底渡船) 在 Escherichia 关口 i 被克隆。用绳子拖的平底渡船向量序列被相应再结合介绍进 MDV 染色体的 US2 地点。包含 BAC 向量的病毒的 DNA 被用来转变 DH10B 的 Escherichia 关口 i 紧张。然后， recombinant 病毒被 recombinant 用绳子拖的平底渡船 DNA 的 transfection 成功地救进主要的鸡胚胎成纤维细胞(CEF ) 。这用绳子拖的平底渡船病毒的克隆被称为 bac-GX0101。当重新组成的病毒被接种进 1-day-old 鸟时，内脏的肿瘤能象 62 d 柱子感染一样早被检测。处于生长能力没有差别，到在 BAC 之间的鸟的致病力导出病毒和它的父母病毒。用绳子拖的平底渡船导出维持的病毒它的 oncogenicity 和抑制免疫力的效果。在结论， GX0101 紧张的完全的染色体成功地被克隆进用绳子拖的平底渡船，传染克隆被救。与强大的用绳子拖的平底渡船操作系统，传染克隆将为进一步在 MDV 的 GX0101 紧张理解插入的 REV-LTR 顺序的功能的角色提供一个有用工具。" @default.
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- W3141959374 date "2009-01-01" @default.
- W3141959374 modified "2023-09-24" @default.
- W3141959374 title "A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity" @default.
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