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• W3141966144 abstract "Economical production of bioethanol from lignocellulosic biomass still faces many technical limita- tions. Cost-effective production of fermentable sugars is still not practical for large-scale production of bioethanol due to high costs of lignocellulolytic enzymes. Therefore, plant molecular farming, where plants are used as biore- actors, was developed for the mass production of cell wall degrading enzymes that will help reduce costs. Subcellular targeting is also potentially more suitable for the accu- mulation of recombinant cellulases. Herein, we generated transgenic tobacco plants (Nicotiana tabacum cv. SR1) that accumulated Thermotoga maritima BglB cellulase, which was driven by the alfalfa RbcsK-1A promoter and con- tained a small subunit of the rubisco complex transit pep- tide. The generated transformants possessed high specific BglB activity and did not show any abnormal phenotypes. Furthermore, we genetically engineered the RbcsK-1A promoter (MRbcsK-1A) and fused the amplification pro- moting sequence (aps )t oMRbcsK-1A promoter to obtain high expression of BglB in transgenic plants. AMRsB plant lines with aps-MRbcsK-1A promoter showed the highest specific activity of BglB, and the accumulated BglB pro- tein represented up to 9.3 % of total soluble protein. When BglB was expressed in Arabidopsis and tobacco plants, the maximal production capacity of recombinant BglB was 0.59 and 1.42 mg/g wet weight, respectively. These results suggests that suitable recombinant expression of cellulases in subcellular compartments such as chloroplasts will contribute to the cost-effective production of enzymes, and will serve as the solid foundation for the future commer- cialization of bioethanol production via plant molecular farming." @default.
• W3141966144 created "2021-04-13" @default.
• W3141966144 creator A5088907714 @default.
• W3141966144 date "2013-01-01" @default.
• W3141966144 modified "2023-09-24" @default.
• W3141966144 title "Improved recombinant cellulase expression in chloroplast of tobacco through promoter engineering and 5 0 amplification promoting sequence" @default.
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• W3141966144 hasPublicationYear "2013" @default.
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