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- W3142091481 abstract "ObjectiveResearch was carried out to study the relationship of tribenuron-methyl with resistance development,embryonic callus growth,regeneration rate,ALS sensitivity,the resistance of regenerated plantlet,bring academic support to the study of resistance of ALS inhibitor herbicides and resistance breeding. MethodEmbryonic callus derived from tobacco (NC-82) were transferred to MS culture medium substrate with different doses of tribenuron-methyl and bred for 12 generations. Result The callus resistance to tribenuron-methyl was enhanced during subculture with resistance index of 1.4,3.6,11.6 and 18.5. LC50 was 4.2,10.8,34.9,55.7 a.i.mg·L-1 respectively with selected doses 0,2,3,5 a.i.mg·L-1 at the 12th generation while ALS sensitivity to tribenuron-methyl was reduced. The speed of tobacco callus resistance development was faster with higher dose. The survival rates of regenerated plantlet treated with higher doses(30 a.i.g.ha-1) of tribenuron-methyl were 5.8%,27.3%,43.8% and 63.3%,and the induced resistance was expressed in the plantlet. During subculture the regeneration rate of callus was reduced and engendered slight resistance,but not related with tribenuron-methyl inducement. ConclusionThe resistance development of tobacco embryonic callus to tribenuron-methyl was very fast,with higher speed in the higher selective press. Tribenuron-methyl obviously restrained the callus growth,changed the callus appearance. At the same time ALS sensitivity to tribenuron-methyl was reduced,and the resistance was expressed in the regenerated plantlet." @default.
- W3142091481 created "2021-04-13" @default.
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- W3142091481 date "2007-01-01" @default.
- W3142091481 modified "2023-09-23" @default.
- W3142091481 title "Inducement of Tribenuron-methyl Resistant Callus and Regeneration of Plantlet in Tobacco" @default.
- W3142091481 hasPublicationYear "2007" @default.
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