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- W3142130038 abstract "Background With high cell doses required for mesenchymal stromal cell (MSC) clinical trials, there is a need to upgrade technologies that facilitate efficient scale up of MSCs for cell therapy. Conventional expansion with 2D culture vessels becomes the bottleneck when large cell dosages are required. Tide Motion bioreactors offer a robust, scalable platform using BioNOC II macrocarriers developed for the production of adherent cells. Methods We evaluated the growth and expansion of bone marrow‐derived MSCs (BM‐MSCs) on the macrocarrier‐based culture system by optimizing key parameters such as cell seeding densities, culturing conditions, and harvesting procedures to achieve optimal cell growth. BM‐MSCs expanded in conventional 2D adherent cultures were seeded into BioNOC II macrocarriers and grown in serum‐containing or serum‐free medium. Results BM‐MSCs attained a maximum cell density of 0.49 ± 0.07 × 10 6 cells/carrier after 12 days of culture in BioNOC II macrocarriers with cell viability > 86% while retaining MSC specific characteristics such as surface marker expression, tri‐lineage differentiation potential, immunosuppressive properties, and potency. Conclusion These results reveal the feasibility of BM‐MSC expansion in the scalable macrocarrier‐based Tide Motion system both under serum and serum‐free conditions and represent an important step for the large‐scale production system of BM‐MSC based cellular therapies." @default.
- W3142130038 created "2021-04-13" @default.
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- W3142130038 date "2021-04-25" @default.
- W3142130038 modified "2023-10-16" @default.
- W3142130038 title "Optimization of culture conditions for human bone marrow‐derived mesenchymal stromal cell expansion in macrocarrier‐based Tide Motion system" @default.
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- W3142130038 doi "https://doi.org/10.1002/biot.202000540" @default.
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