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- W3142286987 abstract "Objective To evaluate killing effects of cytosine deaminase gene mediated by adenovirus vector on human pancreatic cancer cell line Patu 8988.Methods Cytosine Deaminase(CD) gene was cloned into pAdTrack CMV CD,then pAdTrack CMV CD and pAdEasy 1 were recombinated in bacteria.The newly recombinated Ad CD containing green fluorescent protein (GFP) were propagated in 293 cells and purified by cesium chloride gradient centrifugation.Human pancreatic carcinoma cell line 8988 were infected with this virus,then 5 Fc was added,XTT assay was used to estimate relative numbers of viable cells.Results The positive clones were selected by endonuclease to digest the combinatants and the titre of virus containing CD gene was 2×1011 pfu/ml.It was found that significant cytotoxic activities were possessed by 5 Fc for CD gene transduced 8988 cell line,but little effects on the nontransduced pancreatic carcinoma cells.Conclusion CD gene mediated by adenovirus has a high infectivity and is efficient for killing cultured pancreatic carcinoma cells.These data demonstrate utilization of suicide gene prodrug stratege may be effective for combating pancreatic cancer." @default.
- W3142286987 created "2021-04-13" @default.
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- W3142286987 date "2002-01-01" @default.
- W3142286987 modified "2023-09-25" @default.
- W3142286987 title "Killing Effect of Adenoviral Mediated Cytosine Deaminase Gene on Human Pancreatic Cancer Cell Line Patu 8988" @default.
- W3142286987 hasPublicationYear "2002" @default.
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