FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3142410596> ?p ?o ?g. }

• W3142410596 endingPage "ii" @default.
• W3142410596 startingPage "i" @default.
• W3142410596 abstract "Nitric oxide and various by-products including nitrite contribute to tissue injury by forming novel intermediates via redox-mediated nitration reactions. Nitration of unsaturated fatty acids generates electrophilic nitrofatty acids such as 9-nitrooleic acid (9-NO) and 10-nitrooleic acid (10-NO), which are known to initiate intracellular signaling pathways. In these studies, we characterized nitrofatty acid- induced signaling and stress protein expression in mouse keratinocytes. Treatment of keratinocytes with 5-25 μM 9-NO or 10-NO for 6 h upregulated mRNA expression of heat shock proteins (hsp's) 27 and 70; primary antioxidants heme oxygenase-1 (HO-1) and catalase; secondary antioxidants glutathione S-transferase (GST) A1/2, GSTA3, and GSTA4; and Cox-2, a key enzyme in prostaglandin biosynthesis. The greatest responses were evident with HO-1, hsp27, and hsp70. In keratinocytes, 9-NO activated JNK and p38 MAP kinases. JNK inhibition suppressed 9-NO-induced HO-1, hsp27, and hsp70 mRNA and protein expression, whereas p38 MAP kinase inhibition suppressed HO-1. In contrast, inhibition of constitutive expression of Erk1/2 suppressed only hsp70, indicating that 9-NO modulates expression of stress proteins by distinct mechanisms. 9-NO and 10-NO also upregulated expression of caveolin-1, the major structural component of caveolae. Western blot analysis of caveolar membrane fractions isolated by sucrose density centrifugation revealed that HO-1, hsp27, and hsp70 were localized within caveolae after nitrofatty acid treatment of keratinocytes, suggesting a link between induction of stress response proteins and caveolin-1 expression. These data indicate that nitrofatty acids are effective signaling molecules in keratinocytes. Moreover, caveolae seem to be important in the localization of stress proteins in response to nitrofatty acids." @default.
• W3142410596 created "2021-04-13" @default.
• W3142410596 date "2003-10-01" @default.
• W3142410596 modified "2023-09-26" @default.
• W3142410596 title "Free Radical Biology and Medicine" @default.
• W3142410596 cites W129435978 @default.
• W3142410596 cites W1481938993 @default.
• W3142410596 cites W1643087207 @default.
• W3142410596 cites W1653031648 @default.
• W3142410596 cites W1963812198 @default.
• W3142410596 cites W1971756409 @default.
• W3142410596 cites W1973019265 @default.
• W3142410596 cites W1976919258 @default.
• W3142410596 cites W1977229794 @default.
• W3142410596 cites W1980038357 @default.
• W3142410596 cites W1982380290 @default.
• W3142410596 cites W1984981225 @default.
• W3142410596 cites W1987622462 @default.
• W3142410596 cites W1992991540 @default.
• W3142410596 cites W1998364135 @default.
• W3142410596 cites W1999554896 @default.
• W3142410596 cites W2000636708 @default.
• W3142410596 cites W2002518365 @default.
• W3142410596 cites W2004517091 @default.
• W3142410596 cites W2016532893 @default.
• W3142410596 cites W2018089147 @default.
• W3142410596 cites W2021737033 @default.
• W3142410596 cites W2027694461 @default.
• W3142410596 cites W2027815586 @default.
• W3142410596 cites W2031770758 @default.
• W3142410596 cites W2032190558 @default.
• W3142410596 cites W2037126186 @default.
• W3142410596 cites W2037404930 @default.
• W3142410596 cites W2038379033 @default.
• W3142410596 cites W2041591721 @default.
• W3142410596 cites W2049814486 @default.
• W3142410596 cites W2051410138 @default.
• W3142410596 cites W2055706314 @default.
• W3142410596 cites W2057514934 @default.
• W3142410596 cites W2059524588 @default.
• W3142410596 cites W2060299210 @default.
• W3142410596 cites W2060732131 @default.
• W3142410596 cites W2061372674 @default.
• W3142410596 cites W2061656219 @default.
• W3142410596 cites W2061676392 @default.
• W3142410596 cites W2063461543 @default.
• W3142410596 cites W2072369991 @default.
• W3142410596 cites W2072925339 @default.
• W3142410596 cites W2085713699 @default.
• W3142410596 cites W2089345261 @default.
• W3142410596 cites W2093522919 @default.
• W3142410596 cites W2101892154 @default.
• W3142410596 cites W2102824402 @default.
• W3142410596 cites W2104199954 @default.
• W3142410596 cites W2106452970 @default.
• W3142410596 cites W2109621677 @default.
• W3142410596 cites W2109692501 @default.
• W3142410596 cites W2109707262 @default.
• W3142410596 cites W2111094345 @default.
• W3142410596 cites W2111613226 @default.
• W3142410596 cites W2118722165 @default.
• W3142410596 cites W2127036217 @default.
• W3142410596 cites W2127283650 @default.
• W3142410596 cites W2131241278 @default.
• W3142410596 cites W2131902476 @default.
• W3142410596 cites W2135022152 @default.
• W3142410596 cites W2139196095 @default.
• W3142410596 cites W2141737447 @default.
• W3142410596 cites W2142094544 @default.
• W3142410596 cites W2144326073 @default.
• W3142410596 cites W2145760315 @default.
• W3142410596 cites W2147759695 @default.
• W3142410596 cites W2148464651 @default.
• W3142410596 cites W2149727316 @default.
• W3142410596 cites W2151184650 @default.
• W3142410596 cites W2155512285 @default.
• W3142410596 cites W2159069352 @default.
• W3142410596 cites W2161464421 @default.
• W3142410596 cites W2161481261 @default.
• W3142410596 cites W2161860465 @default.
• W3142410596 cites W2162194599 @default.
• W3142410596 cites W2164476520 @default.
• W3142410596 cites W2168398545 @default.
• W3142410596 cites W2168601768 @default.
• W3142410596 cites W2170920594 @default.
• W3142410596 cites W2178113031 @default.
• W3142410596 cites W2317224349 @default.
• W3142410596 doi "https://doi.org/10.1016/s0891-5849(03)00556-2" @default.
• W3142410596 hasPublicationYear "2003" @default.
• W3142410596 type Work @default.
• W3142410596 sameAs 3142410596 @default.
• W3142410596 citedByCount "0" @default.
• W3142410596 crossrefType "journal-article" @default.
• W3142410596 hasConcept C185592680 @default.
• W3142410596 hasConcept C70721500 @default.
• W3142410596 hasConcept C71924100 @default.
• W3142410596 hasConcept C86803240 @default.
• W3142410596 hasConceptScore W3142410596C185592680 @default.

• next