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- W3142425193 abstract "Saccharomyces cerevisiae Pif1p helicase 是从酵母被保存到人的 Pif1 亚科的成立成员。酵母 PIF1 基因的潜在的人的相当或相同的事物从 Hek293 房间线的 cDNA 图书馆被克隆。这里，我们描述了 S-transferase (GST ) 熔化了的谷胱甘肽的一个纯化过程 N 终端从酵母截断了人的 Pif1 蛋白质(hPif1deltaN ) 并且描绘了它的 ATP 水解作用活动的酶的动力学。人的 Pif1 的 ATPase 活动依赖于二价的阳离子，例如 Mg2+ ， Ca2+ 和搁浅单人赛的 DNA。为为 ATPase 活动的 ATP 的 Km 是约 200 microM。因为 ATPase 活动为 hPif1 的 helicase 活动是必要的，这些结果将在 hPif1 上便于进一步的调查。" @default.
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- W3142425193 date "2006-01-01" @default.
- W3142425193 modified "2023-09-23" @default.
- W3142425193 title "Characterization of ATPase Activity of Recombinant Human Pif1" @default.
- W3142425193 hasPublicationYear "2006" @default.
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