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- W3142443480 abstract "Erythropoietin (EPO) controls the production of red blood cells, so it is important to maintain high levels of EPO activity and half-life. Here, we modified glycosylation sites in human erythropoietin (HuEPO) gene, resulting in proteins with addition of 1-4 glycosylation sites. The modi- fied gene was introduced into CHO cells. The expressed EPO analogs were analyzed by SDS-PAGE. Half-life of the ana- logs was determined by sialic acid content test. In vivo potencies of analogs were evaluated by reticulocyte count and haematocrit level. The metabolic clearance of recombinant human erythropoietin (rHuEPO) and its analogs were deter- mined by EPO immunoradiometrics assay. We have shown that the carbohydrate content in modified EPO molecules is increased. The modified EPO, (Val 3 Asn 4 Thr 6 Asn 30 Thr 32 Val 87 Asn 88 Thr 90 )EPO, increases 3.3 times in elimination half-life, 2.1 times in activity and prolongs 2 days functional time in vivo in comparison to rHuEPO. These findings suggest that the addition of glycosylation sites in EPO enhances half-life and biological activity of EPO, duration of action of EPO anlogues positively correlated with the number of glycosylated sites, while addition of 4 glycosylation sites does not further enhance the erythropoietic potency." @default.
- W3142443480 created "2021-04-13" @default.
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- W3142443480 date "2010-01-01" @default.
- W3142443480 modified "2023-09-26" @default.
- W3142443480 title "Glycosylation-modified erythropoietin with improved half-life and biological activity" @default.
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