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- W3142536573 abstract "【Objective】The present study was conducted to identify and select miRNA genes related to xylon formation of Eucalyptus grandis in order to lay a theoretical foundation on genetic quality improvement of Eucalyptus grandis variety.【 Method 】 Prediction of stem-loop structure of pre-miRNAs was run on the web-based software psRobot using 3228 miRNA sequences of all plants from mirBase database.Non-miRNA sequences were removed by BLAST search against rfam and pfam database.The characteristics of base composition in miRNA sequences and their precursors were analyzed by Bioedit software.【Result 】Among 3228 miRNA sequences,341 miRNA precursor sequences and 129 mature miRNAs were found,which belong to 28 different miRNA familes.The predicted miRNA length of Eucalyptus grandis ranged from 18 to 23 bp,and the miRNAs of 21 bp in length was at most(76).The miRNA precursor ranged from 71 to 221 bp,and the average was 123 bp.Nucleotide bias of miRNA was found not only in miRNA mature sequences,but also in flanking sequences.Comparative sequence analysis of mature miRNA sequences,it showed that uracil was the dominant at the 1stbase of the 5'-end,which was up to 62.8%,while cytosine is dominant at the 19 thbase.At the same time,the frequency of G was only 9.1% at the 1stbase in miRNA downstream flanking sequences.Target gene prediction results showed that 41 miRNAs were considered as regulate gene for xylon formation.Out of which,34 miRNAs may be the transcription factors,viz.,ARF,HD-ZIPIII,KAN,MYB and NAC;while 10 miRNAs were involved in enzyme of xylon formation,which mainly regulate cinnamyl alcohol dehydrogenase,peroxidase and cellulose synthase,etc.【Conclusion】There are 41 miRNAs related to xylon formation of Eucalyptus grandis,which would be applied on improvement of genetic quality." @default.
- W3142536573 created "2021-04-13" @default.
- W3142536573 creator A5050017025 @default.
- W3142536573 date "2014-01-01" @default.
- W3142536573 modified "2023-09-27" @default.
- W3142536573 title "Bioinformatic analysis of microRNA and their target genes in Eucalyptus grandis" @default.
- W3142536573 hasPublicationYear "2014" @default.
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