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- W3142540966 abstract "Objective To investigate the regulation of CD4+CD25+ T cells (Tregs) on macropaghs in deciduas in unexplained recurrent spontaneous abortion (URSA) patients. Methods Monocytes and Tregs were isolated from decidua of URSA (n=15) and normal,early pregnant women (n=15) by magnetic cell separation and co-cultured for 6 days. The regulation of Tregs on macrophages was assessed in vitro co-cultures (with or without anti-TGF-β and transwell experiments). Expression of MφCD80,CD86,IL-10 and IFN-γ was measured by FCM or ELISA.Results The expression of MφCD80,CD86 were higher whereas the expression of MφIL-10 was lower in decidua in URSA patients compared to controls; while the expression of MφIFN-γ did not differ between the two groups. In vitro,Tregs could inhibit the expression of MφCD80,CD86 and IFN-γ; increase the expression of MφIL-10. Tregs,as incubated in the medium contained TGF-β or in the transwell system,would lost its regulatory ability. Conclusion The regulatory activity of Tregs to Mφs are decreased in unexplained RSA patients." @default.
- W3142540966 created "2021-04-13" @default.
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- W3142540966 date "2010-01-01" @default.
- W3142540966 modified "2023-09-26" @default.
- W3142540966 title "The regulation of decidual regulatory T cells on macrophages in unexplained recurrent spontaneous abortion patients" @default.
- W3142540966 hasPublicationYear "2010" @default.
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