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• W3142775443 abstract "Traditional approaches to the directed evolution of genes of interest (GOIs) place constraints on the scale of experimentation and depth of evolutionary search reasonably achieved. Engineered genetic systems that dramatically elevate the mutation of target GOIs in vivo relieve these constraints by enabling continuous evolution, affording new strategies in the exploration of sequence space and fitness landscapes for GOIs. We describe various in vivo hypermutation systems for continuous evolution, discuss how different architectures for in vivo hypermutation facilitate evolutionary search scale and depth in their application to problems in protein evolution and engineering, and outline future opportunities for the field." @default.
• W3142775443 created "2021-04-13" @default.
• W3142775443 creator A5035752038 @default.
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• W3142775443 date "2021-10-01" @default.
• W3142775443 modified "2023-10-10" @default.
• W3142775443 title "Systems for in vivo hypermutation: a quest for scale and depth in directed evolution" @default.
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• W3142775443 doi "https://doi.org/10.1016/j.cbpa.2021.02.008" @default.
• W3142775443 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8464631" @default.
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• W3142775443 hasPublicationYear "2021" @default.
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