FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3142824402> ?p ?o ?g. }

• W3142824402 abstract "Introduction. Cholix toxin (ChxA) is an ADP-ribosylating exotoxin produced by Vibrio cholerae . However, to date, there is no quantitative assay available for ChxA, which makes it difficult to detect and estimate the level of ChxA produced by V. cholerae . Hypothesis/Gap Statement. It is important to develop a reliable and specific quantitative assay to measure the production level of ChxA, which will help us to understand the role of ChxA in V. cholerae pathogenesis. Aim. The aim of this study was to develop a bead-based sandwich ELISA (bead-ELISA) for the quantification of ChxA and to evaluate the importance of ChxA in the pathogenesis of V. cholerae infection. Methodology. Anti-rChxA was raised in New Zealand white rabbits, and Fab-horse radish peroxidase conjugate was prepared by the maleimide method to use in the bead-ELISA. This anti-ChxA bead-ELISA was applied to quantify the ChxA produced by various V. cholerae strains. The production of ChxA was examined in different growth media such as alkaline peptone water (APW), Luria-Bertani broth and AKI. Finally, the assay was evaluated using a mouse lethality assay with representative V. cholerae strains categorized as low to high ChxA-producers based on anti-ChxA bead-ELISA. Results. A sensitive bead-ELISA assay, which can quantify from 0.6 to 60 ng ml −1 of ChxA, was developed. ChxA was mostly detected in the extracellular cell-free supernatant and its production level varied from 1.2 ng ml −1 to 1.6 µg ml −1 . The highest ChxA production was observed when V. cholerae strains were cultured in LB broth, but not in APW or AKI medium. The ChxA-producer V. cholerae strains showed 20–80 % lethality and only the high ChxA II-producer was statistically more lethal than a non-ChxA-producer, in the mice model assay. ChxA I and II production levels were not well correlated with mice lethality, and this could be due to the heterogeneity of the strains tested. Conclusion. ChxA I to III was produced mostly extracellularly at various levels depending on strains and culture conditions. The bead-ELISA developed in this study is useful for the detection and quantification of ChxA in V. cholerae strains." @default.
• W3142824402 created "2021-04-13" @default.
• W3142824402 creator A5008975724 @default.
• W3142824402 creator A5026765572 @default.
• W3142824402 creator A5026921490 @default.
• W3142824402 creator A5044793023 @default.
• W3142824402 creator A5052309984 @default.
• W3142824402 creator A5061788299 @default.
• W3142824402 creator A5084196556 @default.
• W3142824402 date "2021-04-08" @default.
• W3142824402 modified "2023-09-27" @default.
• W3142824402 title "Quantification of Vibrio cholerae cholix exotoxin by sandwich bead-ELISA" @default.
• W3142824402 cites W1578171216 @default.
• W3142824402 cites W1830243588 @default.
• W3142824402 cites W1972038449 @default.
• W3142824402 cites W1996826903 @default.
• W3142824402 cites W2001304636 @default.
• W3142824402 cites W2033221441 @default.
• W3142824402 cites W2042220177 @default.
• W3142824402 cites W2064788874 @default.
• W3142824402 cites W2091201192 @default.
• W3142824402 cites W2108301297 @default.
• W3142824402 cites W2109189423 @default.
• W3142824402 cites W2111538738 @default.
• W3142824402 cites W2119009760 @default.
• W3142824402 cites W2129214779 @default.
• W3142824402 cites W2160473668 @default.
• W3142824402 cites W2165689633 @default.
• W3142824402 cites W2167996960 @default.
• W3142824402 doi "https://doi.org/10.1099/jmm.0.001311" @default.
• W3142824402 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33830907" @default.
• W3142824402 hasPublicationYear "2021" @default.
• W3142824402 type Work @default.
• W3142824402 sameAs 3142824402 @default.
• W3142824402 citedByCount "0" @default.
• W3142824402 crossrefType "journal-article" @default.
• W3142824402 hasAuthorship W3142824402A5008975724 @default.
• W3142824402 hasAuthorship W3142824402A5026765572 @default.
• W3142824402 hasAuthorship W3142824402A5026921490 @default.
• W3142824402 hasAuthorship W3142824402A5044793023 @default.
• W3142824402 hasAuthorship W3142824402A5052309984 @default.
• W3142824402 hasAuthorship W3142824402A5061788299 @default.
• W3142824402 hasAuthorship W3142824402A5084196556 @default.
• W3142824402 hasConcept C109386097 @default.
• W3142824402 hasConcept C134306372 @default.
• W3142824402 hasConcept C159985019 @default.
• W3142824402 hasConcept C182179738 @default.
• W3142824402 hasConcept C185592680 @default.
• W3142824402 hasConcept C192562407 @default.
• W3142824402 hasConcept C197336794 @default.
• W3142824402 hasConcept C2777006276 @default.
• W3142824402 hasConcept C2777367657 @default.
• W3142824402 hasConcept C2777637488 @default.
• W3142824402 hasConcept C2778643871 @default.
• W3142824402 hasConcept C2778723905 @default.
• W3142824402 hasConcept C28406088 @default.
• W3142824402 hasConcept C33923547 @default.
• W3142824402 hasConcept C523546767 @default.
• W3142824402 hasConcept C54355233 @default.
• W3142824402 hasConcept C55493867 @default.
• W3142824402 hasConcept C86803240 @default.
• W3142824402 hasConcept C89423630 @default.
• W3142824402 hasConceptScore W3142824402C109386097 @default.
• W3142824402 hasConceptScore W3142824402C134306372 @default.
• W3142824402 hasConceptScore W3142824402C159985019 @default.
• W3142824402 hasConceptScore W3142824402C182179738 @default.
• W3142824402 hasConceptScore W3142824402C185592680 @default.
• W3142824402 hasConceptScore W3142824402C192562407 @default.
• W3142824402 hasConceptScore W3142824402C197336794 @default.
• W3142824402 hasConceptScore W3142824402C2777006276 @default.
• W3142824402 hasConceptScore W3142824402C2777367657 @default.
• W3142824402 hasConceptScore W3142824402C2777637488 @default.
• W3142824402 hasConceptScore W3142824402C2778643871 @default.
• W3142824402 hasConceptScore W3142824402C2778723905 @default.
• W3142824402 hasConceptScore W3142824402C28406088 @default.
• W3142824402 hasConceptScore W3142824402C33923547 @default.
• W3142824402 hasConceptScore W3142824402C523546767 @default.
• W3142824402 hasConceptScore W3142824402C54355233 @default.
• W3142824402 hasConceptScore W3142824402C55493867 @default.
• W3142824402 hasConceptScore W3142824402C86803240 @default.
• W3142824402 hasConceptScore W3142824402C89423630 @default.
• W3142824402 hasFunder F4320334764 @default.
• W3142824402 hasIssue "4" @default.
• W3142824402 hasLocation W31428244021 @default.
• W3142824402 hasOpenAccess W3142824402 @default.
• W3142824402 hasPrimaryLocation W31428244021 @default.
• W3142824402 hasRelatedWork W1758049144 @default.
• W3142824402 hasRelatedWork W2001360571 @default.
• W3142824402 hasRelatedWork W2042220177 @default.
• W3142824402 hasRelatedWork W2056143051 @default.
• W3142824402 hasRelatedWork W2110312205 @default.
• W3142824402 hasRelatedWork W2121963792 @default.
• W3142824402 hasRelatedWork W2357404035 @default.
• W3142824402 hasRelatedWork W2462803464 @default.
• W3142824402 hasRelatedWork W2884499239 @default.
• W3142824402 hasRelatedWork W4249278485 @default.
• W3142824402 hasVolume "70" @default.
• W3142824402 isParatext "false" @default.
• W3142824402 isRetracted "false" @default.
• W3142824402 magId "3142824402" @default.

• next