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- W3142834951 abstract "BACKGROUND OBJECTIVE: Up to now,there is no efficient immunotherapy for hepatocellular carcinoma (HCC). Dendritic cell (DC) vaccine could be a potential tool for HCC immunotherapy. This study was to evaluate the effect of dendritic cells (DCs) transfected with recombinant plasmid bearing hepatitis B virus surface antigen (HBsAg) gene, and the capability of generating specific cytotoxic T lymphocytes (CTL) response against HepG2.2.15 in vitro, which were induced by genetically modified DCs. METHODS: After cultured for 5 days, the DCs were transfected with pCR3.1 S by liposome. The HBsAg gene expression on pCR3.1 transfected DCs was identified by Western blot analysis, and immunofluorescence methods. The cytotoxicity against HepG2.2.15, which were induced by DCs, was tested by MTT assay. RESULTS: DCs up regulated the expression of CD1a (55.0%), CD11c (98.6%), CD86 (86.1%), CD80 (66.1%), and HLA DR (88.9%) after cultured for 5 days. Indirect immunofluo rescence,and Western blot analysis showed that HBsAg gene was expressed on transfected DCs. The death rates of HepG2.2.15 cells induced by DCs transfected with pCR3.1 S were (52.3±2.8)%(E∶T=5∶1), (64.6±2.4)%(10∶1), (78.8±2.6) (20∶1), (82.1±2.4)%(40∶1), while the pCR3.1 transfected and non transfected DCs only induced relatively lower cytotoxicity (P 0.05, n=4). CONCLUSION: DCs transfected with recombined plasmid expressed HBsAg efficiently, and the genetically modified DCs evoke a higher CTL response in vitro." @default.
- W3142834951 created "2021-04-13" @default.
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- W3142834951 date "2004-01-01" @default.
- W3142834951 modified "2023-09-25" @default.
- W3142834951 title "Cytotoxicity Induced by HBsAg Gene Modified Dendritic Cells against Hepatocellular Carcinoma Cell HepG2.2.15" @default.
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