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- W3142956211 abstract "Objective To transfect rat adipose tissue-derived adult stem cells(ADSCs) with the pacemaker gene of human hyperpolarization-activated cyclic nucleotide-gated ion channel 2(hHCN2),and observe its feasibility of functioning as pacemaker cells.Methods First of all,healthy ADSCs were harvested and transfected with hHCN2 gene.Thereafter,we detected the expression of hHCN2 gene and protein by quantitative real-time PCR(qRT-PCR),Western blot analysis and immunofluorescence cytochemistry and measured the electrophysiological properties and recorded the inward currents using patch clamp techniques.In addition,we mixed and co-cultured the transfected ADSCs and rat cadiomyocytes to observe the influence on the spontaneous beating rate of cadiomyocytes.Results Compared with the control ADSCs,the transfected ADSCs expressed the higher level of hHCN2 gene and protein(P0.05),and they could produce pacemaker current.When co-cultured with rat ventricular myocytes,they could accelerate the spontaneous beating rate of the ventricular myocytes(P0.05).Conclusion After transfected with hHCN2 gene,ADSCs can work as pacemaker-like cells,which provides materials for the research of biological pacemakers." @default.
- W3142956211 created "2021-04-13" @default.
- W3142956211 creator A5085180031 @default.
- W3142956211 date "2013-01-01" @default.
- W3142956211 modified "2023-09-24" @default.
- W3142956211 title "Adipose tissue-derived adult stem cells transfected with the gene of hyperpolarizationactivated cyclic nucleotide-gated ion channel 2 differentiated into pacemaker-like cells" @default.
- W3142956211 hasPublicationYear "2013" @default.
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