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- W3143014606 abstract "转基因的 sweetpotato 的提高的茎线虫抵抗(cv。Lizixiang ) 用 Oryzacystatin 被完成 -- 我(OCI ) 有 Agrobacterium 的基因调停 tumefaciens 的转变。A。tumefaciens 紧张 EHA105 与 OCI 基因， gusA 基因和 hptII 基因怀有二进制向量 pCAMBIA1301。选择文化用 25 mg L 被进行 ? 1 hygromycin。1 715 植物的一个总数被生产从接种 1 经由体的 embryogenesis 的 Lizixiang 的 450 个房间总数。随机取样的通常认为的转基因的植物的 GUS 试金和 PCR 分析证明他们中的 90.54% 个是转基因的植物。显著地展出的转基因的植物与茎线虫由域评估与 untransformed 控制植物相比提高了抵抗到茎线虫。进抵抗转基因的植物的染色体的 OCI 基因的稳定的集成被南部的污点分析证实，并且综合 OCI 基因的拷贝数字从 1 ～ 4 。在茎的 Transgene overexpression 线虫抵抗的植物被量的即时 PCR 分析表明。这研究为在 sweetpotato 改进茎线虫抵抗提供一个方法。" @default.
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- W3143014606 date "2011-01-01" @default.
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- W3143014606 title "Enhanced Stem Nematode Resistance of Transgenic Sweetpotato Plants Expressing Oryzacystatin-I Gene" @default.
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