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- W3143037811 abstract "To construct a TK -/gG -mutant of pseudorabies virus, the gG detected transfer vector pUSKKBB and genomic DNA of pseudorabies virus TK -/gG -/LacZ + were co transfected into IBRS 2 cells. Transfection progeny were plated onto PK 15 cells and incubated for 2 days under methylcellulose. Then the overlay was removed and replaced by 1% low melting point agarose in DMEM supplemented with 150μg/mL X gal. After 2 days, white plaques were screened for and purified 4 times. By PCR amplification of gG deleted gene and LacZ gene, a recombinant virus with TK -/gG -phenotype was confirmed. Sequence of the PCR product revealed that there were 1,176 bp detection in gG gene of the PRV TK -/gG -mutant. Amplifying the gG deleted gene of different generations of the TK -/gG -mutant showed that the mutant was stable within PK 15 cells. TCID 50 assay indicated that the recombinant virus grows well on PK 15 cells. The mice immunized with the TK -/gG -virus showed no sign of abnormality. As a control, all mice inoculated with PRV strain died from the infection. All mice that received TK -/gG -survived after a lethal PRV challenge. However none of the mice injected with phosphate buffer saline (PBS) survived from the challenge. The above results demonstrated that the recombinant virus could be a candidate marker vaccine strain for eradicating pseudorabies in pig herds." @default.
- W3143037811 created "2021-04-13" @default.
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- W3143037811 date "2004-01-01" @default.
- W3143037811 modified "2023-09-25" @default.
- W3143037811 title "Construction and Characterization of a Pseudorabies Virus TK~-/gG~- Mutant" @default.
- W3143037811 hasPublicationYear "2004" @default.
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