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- W3143065865 abstract "To establish reference values of various immunop henotypic markers in B lymp hocyte population in healt hy Chinese adults and build background information for accurate interpretation of B cell immunop henotyping data in clinical practice , perip heral blood from 41 healt hy adults were collected separately into test tubes containing ED TA2K2 and stored in room temperature no more t han 24 hours before analysis. Whole blood lysis technique and multiparameter flow cytometry were applied to immunop henotype B cells gated on CD19/ SSC dot2plot . The results showed t hat CD22 ,CD20 , CD62L ,CD40 , CD24 , CD79b , CD79a , and FMC 27 were almost positive in t he circulating B cell population , whereas CD11a ,CD80 ,CD103 , CD10 , CD40L , CD54 , CD95L , CD86 ,and CD95 were almost ne gative in t he perap heral blood B lymp hocytes. CD18 ,CD44 ,CD23 ,CD5 ,CD11c and CD43 werepositive in different B cell subpopulations. 78 % of B cells were IgD positive and ratioκ/λ was 1. 26. The significance of all t hese markers in t he differential diagnosis of lymp hoproliferative diseases was discussed. The conclusion is t hat it is necessary to consider t he qualitative and quantitative levels of expression of various markers in normal B cell population in order to accurately interpret t he pat hologcal immunop henotypic data in clinical practice. It is also important to note t he immunotypic differences of B cells between" @default.
- W3143065865 created "2021-04-13" @default.
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- W3143065865 date "2003-01-01" @default.
- W3143065865 modified "2023-09-24" @default.
- W3143065865 title "Immunophenotypic Characterization of Normal Peripheral Blood B Lymphocyte by Flow Cytometry : Reference f or Diagnosis of Chronic B Cell Leukemia/ Lymphoma" @default.
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