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- W3143071570 abstract "Objective To establish a method for the simultaneous separation and determination of 11 kinds of organic acids in black tea by reversed-phase high performance liquid chromatography on a polar-enhanced C_(18) column.Methods Separation was achieved using an ACCHROM XAqua C_(18) column(4.6mm×150 mm,5 μm),under the gradient elution with the mobile phase of acetonitrile and 0.1%(v:v) formic acid/water,1.0 mL/min,25 ℃ and detection at 214 nm.Results The separation was easily completed in 13 min and the recoveries of 11 kinds of organic acids were from 92.07% to 101.64%,with the relative standard deviation between 0.54%~1.93%,and the coefficients of correlation were not less than 0.9986.Six black tea samples from different districts were determined,the organic acids contents of the sample from Fengqing,Yunnan was the highest(668.62 mg/L),while Ceylon tea sample was the lowest(386.67 mg/L);the oxalic acid occupied the highest ratio in black tea organic acids and varied from 119.67~193.18 mg/L in 6 samples,and the contents fluctuation of lactic acid and acetic acid were relatively larger in 6 samples as 0~193.43 mg/L and36.62~193.98 mg/L,respectively.Conclusion This experiment has established a relatively accurate,efficient,convenient detection technology and method of organic acids in tea,using the method to detect organic acid contents in black tea from different regions.It provided some reference data for sensory evaluation of black tea quality and flavor and improving the processing technology by analyzing and comparing the differences of organic acid group in different black tea." @default.
- W3143071570 created "2021-04-13" @default.
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- W3143071570 date "2014-01-01" @default.
- W3143071570 modified "2023-09-23" @default.
- W3143071570 title "Simultaneous separation and determination of organic acids in black tea by high performance liquid chromatography using polar-enhanced C_(18) column" @default.
- W3143071570 hasPublicationYear "2014" @default.
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