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• W3143080259 abstract "A newly isolated Bacillus gibsonii, designated as S-2 (CGMCC1215), was cultivated for production of alkaline pectinases utilizing sugar beet pulp as growth substrate. Purification of three alkaline endopolygalacturonases (endoPGs) from the crude pectinases extract was carried out by ultra-filtration, ammonium sulphate fractionation and ion-exchange chromatography, and their enzyme activities characterized. The three purified alkaline endoPGs, designated as S-I, S-II, and S-III, had a molecular weight about 38 kDa as determined by SDS−PAGE. The Km value and optimal temperature for optimal enzyme activities of S-I, S-II and S-III were 1.2 mg/mL and 60°C, 0.9 mg/mL and 55°C, 1.1 mg/mL and 60°C, respectively. Their best performances were given at an optimal pH 10.5, and sodium polygalacturonate was found to be the best substrate. The isoelectric points of S-I, S-II and S-III were 5.4, 7.4, and 8.2, respectively. Surfactants of Tween-80 and Tween-20 and metal ions such as Mg and Ca stimulated the activity of S-I, S-II and S-III, whereas S-III was inhibited by Ca, and Mn and Zn ions inhibited the activity of the three enzymes." @default.
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• W3143080259 date "2008-01-01" @default.
• W3143080259 modified "2023-09-23" @default.
• W3143080259 title "Purification and Characterization of Three Alkaline Endopolygalacturonases from a Newly Isolated Bacillus gibsonii" @default.
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