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- W3143119492 abstract "【Objective】 The research determined the classification and systemic evolution status of entomopathogenic bacteria YL001 and YL002,and laid the foundation for further research,development and application of such biological resource.【Method】 Molecular identification was done for YL001 and YL002 strain,the complete 16S rRNA sequence was amplified and cloned;Sequence and phylogenetical analysis were studied with parameters determination.【Result】 Strains YL001 and YL002 formed a monophyletic clade with strains of X.nematophilus with sequence homology outweighing 99% and the sequence homology to genus Photorhabdus outweighing 94%.The lag,logarithmic,stationary and contabescence phase of the two bacteria were 0-6,6-18,18-66 and 66 h respectively;pH of strains YL001 and YL002 reduced to 5.70 and 5.56 at 12 h,respectively,and then rose gradually to 7.74 and 8.07 respectively when culture finished.Glucose content reduced quickly during 0-18 h,and then kept stable.Amido nitrogen content reached the lowest at 12 h and then rose slowly.YL001 exhibited the highest inhibitory effects at 54 h and YL002 exhibited highest inhibitory effects at 42 and 66 h respectively on B.cirerea and B.subtilis.【Conclusion】 Strains YL001 and YL002 belonged to X.nematophilus.Cultivation characteristics of strains YL001 and YL002 were in accordance with the general disciplines of bacteria growth and metabolism,and the optimum fermentation period was 54 and 66 h respectively." @default.
- W3143119492 created "2021-04-13" @default.
- W3143119492 creator A5074819283 @default.
- W3143119492 date "2008-01-01" @default.
- W3143119492 modified "2023-09-25" @default.
- W3143119492 title "Molecular identification and cultivation characteristics of entomopathogenic bacteria" @default.
- W3143119492 hasPublicationYear "2008" @default.
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