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- W3143148137 abstract "Abstract Plastic waste can serve as a feedstock for microbial bioconversion using a chemical/biological hybrid strategy. We developed a polypropylene (PP) upcycling process that coupled pyrolysis with bioconversion by the oleaginous yeast Yarrowia lipolytica. Using virgin PP, we optimized pH, inoculum density, C/N ratio, and osmolarity and increased the fatty acid titer nearly four-fold to 1.9 g L−1, with 41 percent cellular fatty acid content, the highest content reported to date for plastic-to-lipid microbial bioconversion. The highest fatty acid titer was achieved with an inoculum density of 3 (OD 600 nm), pH = 6.0 and C/N ratio of 80:1. Increasing the medium osmolarity by adding sodium chloride adversely affected cell growth and did not improve the fatty acid titer. The maximum fatty acid titer occurred under conditions that balanced cell growth versus lipogenesis. Using postconsumer PP, the fatty acid titer was significantly lower (0.13 g L−1). Overall, the work demonstrates the potential and the challenges associated with microbial bioconversion of plastics." @default.
- W3143148137 created "2021-04-13" @default.
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- W3143148137 date "2021-05-01" @default.
- W3143148137 modified "2023-10-18" @default.
- W3143148137 title "Enhancing polypropylene bioconversion and lipogenesis by Yarrowia lipolytica using a chemical/biological hybrid process" @default.
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- W3143148137 doi "https://doi.org/10.1016/j.jbiotec.2021.03.015" @default.
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